Incubation temperature affects growth and efficacy of white-rot fungi to improve the nutritive value of rice straw

IF 1.3 4区农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Production Science Pub Date : 2024-05-16 DOI:10.1071/an23403

T. T. Hai, A. V. Peer, J. W. Cone, J. T. Schonewille, J. Baars, L. D. Phung, W. H. Hendriks

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引用次数: 0

Abstract

Context A great body of evidence is available on the in vitro efficacy of white-rot fungi (WRF) to degrade lignin in fibre-rich biomass (e.g. wheat straw, wood chips and rice straw (RS)) and improve the biomass’ nutritive value for ruminants. Aims Determining the impact of incubation temperature of three WRF to improve the nutritional value of rice straw. Methods Growth of Ceriporiopsis subvermispora, Lentinula edodes and Pleurotus eryngii on RS for 26 days at the following six temperature regimes: continuous at 24°C, 30°C, 35°C and 40°C, and 3 days at 35°C and 40°C, with subsequent days at 24°C. In a follow-up experiment, improvement in fermentability in buffered rumen fluid of RS treated by the three WRF at 24°C and 30°C for up to 8 weeks was investigated. Key results All three fungi grew at temperatures up to 35°C, with no growth observed at 40°C, with C. subvermispora being more temperature sensitive. There were significant differences in cellulose, hemicellulose and lignin degradation of RS at 24°C and 30°C, with C. subvermispora degrading 69% and 90% of the hemicellulose and lignin respectively at 30°C, greater than at 24°C (55% and 80% respectively). For L. edodes, there were significant differences in cellulose degradation between 24°C and 30°C, with 12% more degradation at 30°C, but not for hemicellulose and lignin. In vitro gas production showed no significant differences between the two incubation temperatures for either of the two fungi. Pleurotus eryngii treatment did not show any improvement in terms of in vitro gas production. Conclusions Treatment of RS with L. edodes and C. subvermispora, but not P. eryngii, is robust and temperature changes will not have a major impact on their efficacy as long as the temperature remains below 30°C. Implications Temperature during the incubation of WRF with rice straw needs to be below 30°C for this biotechnology to be applied in practice.

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孵化温度影响白腐真菌的生长和提高稻草营养价值的功效

背景关于白腐真菌（WRF）降解富含纤维的生物质（如小麦秸秆、木屑和稻草（RS））中的木质素并提高生物质对反刍动物的营养价值的体外功效，已有大量证据。目的确定三种 WRF 培养温度对提高稻草营养价值的影响。方法 Ceriporiopsis subvermispora、Lentinula edodes 和 Pleurotus eryngii 在以下六种温度条件下在 RS 上生长 26 天：连续 24°C、30°C、35°C 和 40°C，35°C 和 40°C 条件下生长 3 天，随后几天在 24°C 条件下生长。在后续实验中，研究了在 24 摄氏度和 30 摄氏度条件下用这三种 WRF 处理 RS 长达 8 周的缓冲瘤胃液发酵性的改善情况。主要结果这三种真菌在最高 35°C 的温度下都能生长，在 40°C 的温度下则无法生长，其中 C. subvermispora 对温度更为敏感。在 24 摄氏度和 30 摄氏度条件下，RS 的纤维素、半纤维素和木质素降解量存在明显差异，亚藜孢属真菌在 30 摄氏度条件下的半纤维素和木质素降解量分别为 69% 和 90%，高于 24 摄氏度条件下的降解量（分别为 55% 和 80%）。对于 L. edodes，24°C 和 30°C 下的纤维素降解量存在显著差异，30°C 下的降解量多 12%，但半纤维素和木质素的降解量没有显著差异。两种真菌的体外产气量在两种培养温度下没有明显差异。Pleurotus eryngii 处理对体外产气量没有任何改善。结论使用 L. edodes 和 C. subvermispora（而非 P. eryngii）处理 RS 是可靠的，只要温度保持在 30°C 以下，温度变化不会对其功效产生重大影响。意义用稻草培养 WRF 时的温度必须低于 30°C，才能将这种生物技术应用于实践。

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来源期刊

Animal Production Science AGRICULTURE, MULTIDISCIPLINARY-

自引率

7.10%

发文量

139

期刊介绍： Research papers in Animal Production Science focus on improving livestock and food production, and on the social and economic issues that influence primary producers. The journal (formerly known as Australian Journal of Experimental Agriculture) is predominantly concerned with domesticated animals (beef cattle, dairy cows, sheep, pigs, goats and poultry); however, contributions on horses and wild animals may be published where relevant. Animal Production Science is published with the endorsement of the Commonwealth Scientific and Industrial Research Organisation (CSIRO) and the Australian Academy of Science.