The impact of autologous platelet-rich plasma supplement on Ossimi ram semen quality and fertility: In vivo fertility of freshly diluted and frozen-thawed spermatozoa

IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Maha S. Salama , Ahmed M. Shehabeldin , Mohey A. Ashour , Lamya Ahmed Alkeridis , Laila A. Al-Shuraym , Samy M. Sayed , Foad Farrag , Mustafa Shukry
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引用次数: 0

Abstract

The study aimed to investigate the effects of using the ram's platelet-rich plasma (PRP) as a supplement on the quality, functionality, antioxidant activity, and in vivo fertilization of freshly diluted and frozen-thawed ram semen. Nine Ossimi rams were involved in the analysis, which comprised 162 ejaculates. Each ejaculate was segmented into five parts and mixed with a Tris-based extender containing varying concentrations of autologous PRP (ranging from 0% to 10%) for both freshly diluted and frozen-thawed semen. The evaluation encompassed sperm viability, motility, kinetics, functional traits, antioxidant enzyme activity, lipid peroxidation content, and in vivo fertility. Results showed that supplementing with 5% PRP in both freshly diluted and frozen-thawed semen led to increased total and progressive motility of sperm, improved sperm kinetics and functional characteristics, and enhanced viability compared to the control groups. Notably, concentrations of PRP at 5% and 7.5% reduced malondialdehyde levels while increasing total antioxidant capacity, glutathione reductase, and superoxide dismutase levels compared to the groups supplemented with 10% PRP and the control groups. Furthermore, the in vivo fertilization rate of ram semen significantly improved with 5% PRP supplementation compared to the control groups for both freshly diluted and frozen-thawed semen.

In conclusion, the study suggests that incorporating 5% autologous PRP into seminal extenders can protect Ossimi ram sperm and enhance in vivo fertility for freshly diluted and frozen-thawed semen.

补充自体富血小板血浆对奥西米公羊精液质量和繁殖力的影响新鲜稀释和冷冻解冻精子的体内繁殖力
该研究旨在探讨使用公羊富血小板血浆(PRP)作为补充剂对新鲜稀释和冷冻解冻公羊精液的质量、功能、抗氧化活性和体内受精率的影响。九只奥西米公羊的162次射精参与了分析。每份射精都被分成五份,并与含有不同浓度(从 0% 到 10% 不等)自体 PRP 的 Tris 型扩展剂混合,用于新稀释的精液和冷冻解冻的精液。评估内容包括精子存活率、活力、动力学、功能特征、抗氧化酶活性、脂质过氧化物含量和体内生育能力。结果表明,与对照组相比,在新鲜稀释精液和冷冻解冻精液中补充 5%的 PRP 可提高精子的总活力和渐进活力,改善精子动力学和功能特性,并提高存活率。值得注意的是,与补充 10% PRP 的组别和对照组相比,浓度为 5% 和 7.5% 的 PRP 可降低丙二醛水平,同时提高总抗氧化能力、谷胱甘肽还原酶和超氧化物歧化酶水平。总之,该研究表明,在精液扩展剂中加入 5%的自体 PRP 可以保护奥西米公羊精子,并提高新鲜稀释和冷冻解冻精液的体内受精率。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Small Ruminant Research
Small Ruminant Research 农林科学-奶制品与动物科学
CiteScore
3.10
自引率
11.10%
发文量
210
审稿时长
12.5 weeks
期刊介绍: Small Ruminant Research publishes original, basic and applied research articles, technical notes, and review articles on research relating to goats, sheep, deer, the New World camelids llama, alpaca, vicuna and guanaco, and the Old World camels. Topics covered include nutrition, physiology, anatomy, genetics, microbiology, ethology, product technology, socio-economics, management, sustainability and environment, veterinary medicine and husbandry engineering.
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