Morphological and molecular characterization of Eimeria haematopusi n. sp. (Apicomplexa: Eimeriidae) in an Australian Pied Oystercatcher (Haematopus longirostris) (Aves: Charadriiformes).

IF 1 4区医学 Q4 PARASITOLOGY

Systematic Parasitology Pub Date : 2024-05-13 DOI:10.1007/s11230-024-10152-5

Jill M Austen, Belinda Brice, Dandan Liu, Huimin Gao, Bruno P Berto, Alireza Zahedi, Aileen Elloit, Rongchang Yang

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Abstract

A novel Eimeria Schneider, 1875 species is described from an Australian pied oystercatcher Haematopus longirostris Vieillot, in Western Australia. The pied oystercatcher was admitted to the Kanyana Wildlife Rehabilitation Centre (KWRC), Perth, Western Australia in a poor body condition, abrasion to its right hock and signs of partial delamination to its lower beak. Investigation into potential medical causes resulted in a faecal sample being collected and screened for gastrointestinal parasites. Unsporulated coccidian oocysts were initially observed in the faeces and identified as Eimeria upon sporulation. The sporulated oocysts (n = 20) are ellipsoidal, 20-21 × 12-13 μm in shape and have thick bi-layered walls which are c.2/3 of the total thickness. Micropyle is present, robust and protruding, and occasionally has a rounded polar body attached to the micropyle. Within the oocyst, a residuum, in addition, two to five polar granules are present. There are four ellipsoidal sporocysts 9-11 × 5-6 μm with flattened to half-moon shaped Stieda bodies. Sub-Stieda body and para-Stieda body are absent. The sporocysts contain sporocyst residuums composed of a few spherules scattered among the sporozoites. Within the sporozoites, anterior and posterior refractile bodies are present, but the nucleus is indiscernible. To further characterise the novel Eimeria species from H. longirostris, molecular analysis was conducted at the 18S ribosomal RNA locus, using PCR amplification and cloning. Two cloned sequences from the novel Eimeria were compared with those from other Eimeria spp. with the highest genetic similarity of 97.6% and 97.2% from Clone 1 and 2, respectively with Eimeria reichenowi (AB544308) from a hooded crane (Grus monacha Temminck) in Japan. Both sequences grouped in a clade with the Eimeria spp. isolated from wetland birds, which include Eimeria paludosa (KJ767187) from a dusky moorhen (Gallinula tenebrosa Gould) in Western Australia, Eimeria reichenowi (AB544308) and Eimeria gruis (AB544336) both from hooded cranes. Based on the morphological and molecular data, this Eimeria sp. is a new species of coccidian parasite and is named Eimeria haematopusi n. sp. after its host H. longirostris.

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澳大利亚斑蛎鹬（Haematopus longirostris）（Aves: Charadriiformes）中的 Eimeria haematopusi n. sp.（Apicomplexa: Eimeriidae）的形态学和分子特征。

本报告描述了一种新型艾美拉氏菌（Eimeria Schneider，1875年），它来自西澳大利亚州的一只澳大利亚灰褐斑蛎鹬（Haematopus longirostris Vieillot）。这只灰褐斑蛎鹬被送到西澳大利亚州珀斯的卡尼亚纳野生动物康复中心（Kanyana Wildlife Rehabilitation Centre，KWRC）时身体状况不佳，右跗关节有擦伤，下喙有部分脱层的迹象。在对可能的病因进行调查后，采集了粪便样本并进行了胃肠道寄生虫筛查。最初在粪便中观察到未孢子化的球虫卵囊，孢子化后被鉴定为艾美耳病。孢子化的卵囊（n = 20）呈椭圆形，大小为 20-21 × 12-13 μm，具有厚厚的双层囊壁，约占总厚度的 2/3。微孔存在，粗壮而突出，偶尔会有一个圆形极体附着在微孔上。卵囊内有残留物，此外还有 2 到 5 个极性颗粒。有四个椭圆形孢子囊，大小为 9-11 × 5-6 μm，孢子囊体扁平至半月形。无子实体和副子实体。孢子囊中含有孢子囊残基，由散布在孢子囊中的几个球体组成。孢子囊内有前折射体和后折射体，但细胞核无法辨认。为了进一步确定来自 H. longirostris 的新型 Eimeria 的特征，利用 PCR 扩增和克隆技术对 18S 核糖体 RNA 位点进行了分子分析。克隆 1 和克隆 2 与来自日本丹顶鹤（Grus monacha Temminck）的 Eimeria reichenowi（AB544308）的遗传相似度最高，分别为 97.6% 和 97.2%。这两个序列与从湿地鸟类中分离出的艾美耳病菌属组成一个支系，其中包括从西澳大利亚的一只昏暗鸬鹚（Gallinula tenebrosa Gould）身上分离出的 Eimeria paludosa (KJ767187)、从丹顶鹤身上分离出的 Eimeria reichenowi (AB544308) 和 Eimeria gruis (AB544336)。根据形态学和分子数据，该Eimeria sp.是一种新的球虫寄生种，并以其宿主H. longirostris命名为Eimeria haematopusi n. sp.。

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来源期刊

Systematic Parasitology 医学-寄生虫学

CiteScore

2.20

自引率

23.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Systematic Parasitology publishes papers on the systematics, taxonomy and nomenclature of the following groups: Nematoda (including plant-parasitic), Monogenea, Digenea, Cestoda, Acanthocephala, Aspidogastrea, Cestodaria, Arthropoda (parasitic copepods, hymenopterans, mites, ticks, etc.), Protozoa (parasitic groups), and parasitic genera in other groups, such as Mollusca, Turbelleria, etc. Systematic Parasitology publishes fully illustrated research papers, brief communications, and fully illustrated major revisions. In order to maintain high standards, all contributors describing new taxa are asked to state clearly where the holotype is deposited and to make paratypes available for examination by the referees. It is recognized that, in some cases, this may cause problems for the authors, but it is hoped that by adhering to this rule authors may be protected against rapid synonymy of their taxa, and the types will be preserved for posterity.