The Nephroprotective Activity of Boesenbergia Rotunda Rhizome by Reducing Creatinine, Urea Nitrogen, Glutamic Pyruvic Transaminase, and Malondialdehyde Levels in the Blood and Attenuating the Expression of Havcr1 (KIM-1), Lcn2 (NGAL), Casp3, and Casp7 Genes in the Kidney Cortex of Cisplatin-Induced Sprague-Dawley Rats.

Q2 Medicine

Journal of Experimental Pharmacology Pub Date : 2024-05-06 eCollection Date: 2024-01-01 DOI:10.2147/JEP.S459483

Dani Sujana, Sri Adi Sumiwi, Nyi Mekar Saptarini, Jutti Levita

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Abstract

Background: Cisplatin chemotherapy induces nephrotoxicity by producing reactive oxygen species, hence, discovering add-on nephroprotective drugs for patients with cancer is challenging. Boesenbergia rotunda has been reported for its antioxidant properties.

Purpose: This study aims to explore the nephroprotective mechanism of the ethanol extract of Boesenbergia rotunda rhizome (EEBR) in cisplatin-induced rats.

Methods: The rats were randomly assigned into 6 groups: the normal control (treated with saline); the negative control (cisplatin-induced without any treatment); the positive control (treated with quercetin 50 mg/kg BW); and 3 treatment EEBR (125 mg/kg BW; 250 mg/kg BW; 500 mg/kg BW) groups for 10 days. The % relative organ weight, kidney histopathology, and nephrotoxicity biomarkers expression were evaluated.

Results: EEBR decreased creatinine, urea nitrogen, glutamic pyruvate transaminase, and malondialdehyde levels in the blood of cisplatin-induced rats. An insignificant increase in GOT was observed in rats treated with the highest dose of EEBR. EEBR did not significantly alter the BW and the % kidney relative weight. An abnormal shape of the Bowman capsule is observed in the negative control group. EEBR reduced the expression of Havcr1 (KIM-1), Lcn2 (NGAL), Casp3, and Casp7 genes in rats' kidneys.

Conclusion: Boesenbergia rotunda could be considered a potential candidate for add-on therapy in cisplatin-treated patients, but further studies are needed to verify its efficacy and safety.

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苧麻通过降低顺铂诱导的 Sprague-Dawley 大鼠血液中肌酐、尿素氮、谷氨酸丙酮酸转氨酶和丙二醛的水平以及减弱 Havcr1 (KIM-1)、Lcn2 (NGAL)、Casp3 和 Casp7 基因在肾皮质中的表达而发挥肾保护作用

背景：顺铂化疗通过产生活性氧而诱发肾毒性，因此，为癌症患者寻找肾脏保护药物具有挑战性。目的：本研究旨在探讨苧麻根茎乙醇提取物（EEBR）对顺铂诱导的大鼠肾脏的保护机制：方法：将大鼠随机分为6组：正常对照组（生理盐水治疗）；阴性对照组（顺铂诱导，无任何治疗）；阳性对照组（槲皮素50 mg/kg体重治疗）；3个EEBR治疗组（125 mg/kg体重；250 mg/kg体重；500 mg/kg体重），连续10天。结果表明：EEBR可降低血肌酐、肾脏组织病理学和肾毒性生物标志物的表达：结果：EEBR降低了顺铂诱导大鼠血液中的肌酐、尿素氮、谷丙转氨酶和丙二醛水平。在接受最高剂量 EEBR 治疗的大鼠中，观察到 GOT 显著增加。EEBR 并未明显改变大鼠的体重和肾脏相对重量的百分比。在阴性对照组中观察到鲍曼囊形状异常。EEBR降低了大鼠肾脏中Havcr1（KIM-1）、Lcn2（NGAL）、Casp3和Casp7基因的表达：结论：苧麻可被视为顺铂治疗患者的一种潜在的附加疗法，但还需要进一步的研究来验证其疗效和安全性。

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