Unveiling the emerging role of curcumin to alleviate ochratoxin A-induced muscle toxicity in grass carp (Ctenopharyngodon idella): in vitro and in vivo studies.

IF 6.3 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Piao Zhao, Lin Feng, Weidan Jiang, Pei Wu, Yang Liu, Hongmei Ren, Xiaowan Jin, Lu Zhang, Haifeng Mi, Xiaoqiu Zhou
{"title":"Unveiling the emerging role of curcumin to alleviate ochratoxin A-induced muscle toxicity in grass carp (Ctenopharyngodon idella): in vitro and in vivo studies.","authors":"Piao Zhao, Lin Feng, Weidan Jiang, Pei Wu, Yang Liu, Hongmei Ren, Xiaowan Jin, Lu Zhang, Haifeng Mi, Xiaoqiu Zhou","doi":"10.1186/s40104-024-01023-6","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Ochratoxin A (OTA), a globally abundant and extremely hazardous pollutant, is a significant source of contamination in aquafeeds and is responsible for severe food pollution. The developmental toxicity of OTA and the potential relieving strategy of natural products remain unclear. This study screened the substance curcumin (Cur), which had the best effect in alleviating OTA inhibition of myoblast proliferation, from 96 natural products and investigated its effect and mechanism in reducing OTA myotoxicity in vivo and in vitro.</p><p><strong>Methods: </strong>A total of 720 healthy juvenile grass carp, with an initial average body weight of 11.06 ± 0.05 g, were randomly assigned into 4 groups: the control group (without OTA and Cur), 1.2 mg/kg OTA group, 400 mg/kg Cur group, and 1.2 mg/kg OTA + 400 mg/kg Cur group. Each treatment consisted of 3 replicates (180 fish) for 60 d.</p><p><strong>Results: </strong>Firstly, we cultured, purified, and identified myoblasts using the tissue block culture method. Through preliminary screening and re-screening of 96 substances, we examined cell proliferation-related indicators such as cell viability and ultimately found that Cur had the best effect. Secondly, Cur could alleviate OTA-inhibited myoblast differentiation and myofibrillar development-related proteins (MyoG and MYHC) in vivo and in vitro and improve the growth performance of grass carp. Then, Cur could also promote the expression of OTA-inhibited protein synthesis-related proteins (S6K1 and TOR), which was related to the activation of the AKT/TOR signaling pathway. Finally, Cur could downregulate the expression of OTA-enhanced protein degradation-related genes (murf1, foxo3a, and ub), which was related to the inhibition of the FoxO3a signaling pathway.</p><p><strong>Conclusions: </strong>In summary, our data demonstrated the effectiveness of Cur in alleviating OTA myotoxicity in vivo and in vitro. This study confirms the rapidity, feasibility, and effectiveness of establishing a natural product screening method targeting myoblasts to alleviate fungal toxin toxicity.</p>","PeriodicalId":64067,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"15 1","pages":"72"},"PeriodicalIF":6.3000,"publicationDate":"2024-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11088780/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Animal Science and Biotechnology","FirstCategoryId":"1089","ListUrlMain":"https://doi.org/10.1186/s40104-024-01023-6","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
引用次数: 0

Abstract

Background: Ochratoxin A (OTA), a globally abundant and extremely hazardous pollutant, is a significant source of contamination in aquafeeds and is responsible for severe food pollution. The developmental toxicity of OTA and the potential relieving strategy of natural products remain unclear. This study screened the substance curcumin (Cur), which had the best effect in alleviating OTA inhibition of myoblast proliferation, from 96 natural products and investigated its effect and mechanism in reducing OTA myotoxicity in vivo and in vitro.

Methods: A total of 720 healthy juvenile grass carp, with an initial average body weight of 11.06 ± 0.05 g, were randomly assigned into 4 groups: the control group (without OTA and Cur), 1.2 mg/kg OTA group, 400 mg/kg Cur group, and 1.2 mg/kg OTA + 400 mg/kg Cur group. Each treatment consisted of 3 replicates (180 fish) for 60 d.

Results: Firstly, we cultured, purified, and identified myoblasts using the tissue block culture method. Through preliminary screening and re-screening of 96 substances, we examined cell proliferation-related indicators such as cell viability and ultimately found that Cur had the best effect. Secondly, Cur could alleviate OTA-inhibited myoblast differentiation and myofibrillar development-related proteins (MyoG and MYHC) in vivo and in vitro and improve the growth performance of grass carp. Then, Cur could also promote the expression of OTA-inhibited protein synthesis-related proteins (S6K1 and TOR), which was related to the activation of the AKT/TOR signaling pathway. Finally, Cur could downregulate the expression of OTA-enhanced protein degradation-related genes (murf1, foxo3a, and ub), which was related to the inhibition of the FoxO3a signaling pathway.

Conclusions: In summary, our data demonstrated the effectiveness of Cur in alleviating OTA myotoxicity in vivo and in vitro. This study confirms the rapidity, feasibility, and effectiveness of establishing a natural product screening method targeting myoblasts to alleviate fungal toxin toxicity.

揭示姜黄素在减轻赭曲霉毒素 A 诱导的草鱼肌肉毒性方面的新作用:体外和体内研究。
背景:赭曲霉毒素 A(OTA)是一种全球含量极高、危害极大的污染物,是水产饲料的重要污染源,也是造成严重食品污染的罪魁祸首。OTA 的发育毒性和天然产物的潜在缓解策略仍不清楚。本研究从96种天然产品中筛选出了对减轻OTA抑制肌细胞增殖效果最好的姜黄素(Cur),并研究了其在体内和体外减轻OTA肌毒性的效果和机制:将初始平均体重为 11.06 ± 0.05 g 的 720 尾健康草鱼幼鱼随机分为 4 组:对照组(不含 OTA 和 Cur)、1.2 mg/kg OTA 组、400 mg/kg Cur 组和 1.2 mg/kg OTA + 400 mg/kg Cur 组。每个处理包括 3 个重复(180 尾鱼),共 60 天:首先,我们用组织块培养法培养、纯化和鉴定了肌母细胞。通过对 96 种物质的初步筛选和再筛选,我们检测了与细胞增殖相关的指标,如细胞活力,最终发现 Cur 的效果最好。其次,Cur 能缓解 OTA 在体内和体外对肌母细胞分化和肌纤维发育相关蛋白(MyoG 和 MYHC)的抑制,改善草鱼的生长性能。然后,Cur 还能促进 OTA 抑制的蛋白质合成相关蛋白(S6K1 和 TOR)的表达,这与 AKT/TOR 信号通路的激活有关。最后,Cur能下调OTA增强蛋白降解相关基因(murf1、foxo3a和ub)的表达,这与抑制FoxO3a信号通路有关:总之,我们的数据证明了 Cur 在减轻体内和体外 OTA 肌毒性方面的有效性。这项研究证实了建立针对成肌细胞的天然产物筛选方法以减轻真菌毒素毒性的快速性、可行性和有效性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
10.30
自引率
0.00%
发文量
822
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信