CLIC4 Function in the Epithelial-Mesenchymal Transition of Epithelial Odontogenic Lesions.

IF 3.2 Q2 PATHOLOGY

Head & Neck Pathology Pub Date : 2024-05-10 DOI:10.1007/s12105-024-01646-1

Mariana Carvalho Xerez, Caio César da Silva Barros, Maurília Raquel de Souto Medeiros, Rodrigo Porpino Mafra, Hévio Freitas de Lucena, Éricka Janine Dantas da Silveira, Antonio de Lisboa Lopes Costa

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引用次数: 0

Abstract

Background: Odontogenic lesions constitute a heterogeneous group of lesions. CLIC4 protein regulates different cellular processes, including epithelial-mesenchymal transition and fibroblast-myofibroblast transdifferentiation. This study analyzed CLIC4, E-cadherin, Vimentin, and α-SMA immunoexpression in epithelial odontogenic lesions that exhibit different biological behavior.

Methods: It analyzed the immunoexpression of CLIC4, E-cadherin, and Vimentin in the epithelial cells, as well as CLIC4 and α-SMA in the mesenchymal cells, of ameloblastoma (AM) (n = 16), odontogenic keratocyst (OKC) (n = 20), and adenomatoid odontogenic tumor (AOT) (n = 8). Immunoexpressions were categorized as score 0 (0% positive cells), 1 (< 25%), 2 (≥ 25% - < 50%), 3 (≥ 50% - < 75%), or 4 (≥ 75%).

Results: Cytoplasmic CLIC4 immunoexpression was higher in AM and AOT (p < 0.001) epithelial cells. Nuclear-cytoplasmic CLIC4 was higher in OKC's epithelial lining (p < 0.001). Membrane (p = 0.012) and membrane-cytoplasmic (p < 0.001) E-cadherin immunoexpression were higher in OKC, while cytoplasmic E-cadherin expression was higher in AM and AOT (p < 0.001). Vimentin immunoexpression was higher in AM and AOT (p < 0.001). Stromal CLIC4 was higher in AM and OKC (p = 0.008). Similarly, α-SMA immunoexpression was higher in AM and OKC (p = 0.037). Correlations in these proteins' immunoexpression were observed in AM and OKC (p < 0.05).

Conclusions: CLIC4 seems to regulate the epithelial-mesenchymal transition, modifying E-cadherin and Vimentin expression. In mesenchymal cells, CLIC4 may play a role in fibroblast-myofibroblast transdifferentiation. CLIC4 may be associated with epithelial odontogenic lesions with aggressive biological behavior.

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CLIC4 在上皮牙源性病变的上皮-间质转化过程中的功能

背景：牙源性病变是一种异质性病变。CLIC4 蛋白调节不同的细胞过程，包括上皮-间质转化和成纤维细胞-肌成纤维细胞的转分化。本研究分析了CLIC4、E-cadherin、Vimentin和α-SMA在表现出不同生物学行为的牙源性上皮病变中的免疫表达：方法：分析了骨髓母细胞瘤（AM）（16 例）、牙源性角化囊肿（OKC）（20 例）和牙源性腺瘤（AOT）（8 例）上皮细胞中的 CLIC4、E-adherin 和 Vimentin 免疫表达，以及间质细胞中的 CLIC4 和 α-SMA 免疫表达。免疫表达分为 0 分（阳性细胞为 0%）、1 分（结果：阳性细胞为 0%）、2 分（结果：阳性细胞为 0%）和 3 分（结果：阳性细胞为 0%）：AM和AOT的细胞质CLIC4免疫表达较高（p 结论：CLIC4似乎能调控牙源性肿瘤的表皮生长：CLIC4似乎能调节上皮-间质转化，改变E-cadherin和Vimentin的表达。在间质细胞中，CLIC4 可能在成纤维细胞-肌成纤维细胞的转分化中发挥作用。CLIC4可能与具有侵袭性生物学行为的上皮性牙本质病变有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Head & Neck Pathology PATHOLOGY-

CiteScore

5.70

自引率

9.50%

发文量

期刊介绍： Head & Neck Pathology presents scholarly papers, reviews and symposia that cover the spectrum of human surgical pathology within the anatomic zones of the oral cavity, sinonasal tract, larynx, hypopharynx, salivary gland, ear and temporal bone, and neck. The journal publishes rapid developments in new diagnostic criteria, intraoperative consultation, immunohistochemical studies, molecular techniques, genetic analyses, diagnostic aids, experimental pathology, cytology, radiographic imaging, and application of uniform terminology to allow practitioners to continue to maintain and expand their knowledge in the subspecialty of head and neck pathology. Coverage of practical application to daily clinical practice is supported with proceedings and symposia from international societies and academies devoted to this field. Single-blind peer review The journal follows a single-blind review procedure, where the reviewers are aware of the names and affiliations of the authors, but the reviewer reports provided to authors are anonymous. Single-blind peer review is the traditional model of peer review that many reviewers are comfortable with, and it facilitates a dispassionate critique of a manuscript.