Diogo Milani, Ana Elisa Gasparotto, Vilma Loreto, Dardo A Martí, Diogo C Cabral-de-Mello
{"title":"Chromosomal and genomic analysis suggests single origin and high molecular differentiation of the B chromosome of <i>Abracris flavolineata</i>.","authors":"Diogo Milani, Ana Elisa Gasparotto, Vilma Loreto, Dardo A Martí, Diogo C Cabral-de-Mello","doi":"10.1139/gen-2023-0122","DOIUrl":null,"url":null,"abstract":"<p><p>Supernumerary chromosomes (B chromosomes) have been an intriguing subject of study. Our understanding of the molecular differentiation of B chromosomes from an interpopulation perspective remains limited, with most analyses involving chromosome banding and mapping of a few sequences. To gain insights into the molecular composition, origin, and evolution of B chromosomes, we conducted cytogenetic and next-generation sequencing analysis of the repeatome in the grasshopper <i>Abracris flavolineata</i> across various populations. Our results unveiled the presence of B chromosomes in two newly investigated populations and described new satellite DNA sequences. While we observed some degree of genetic connection among <i>A. flavolineata</i> populations, our comparative analysis of genomes with and without B chromosomes provided evidence of two new B chromosome variants. These variants exhibited distinct compositions of various repeat classes, including transposable elements and satellite DNAs. Based on shared repeats, their chromosomal location, and the C-positive heterochromatin content on the B chromosome, these variants likely share a common origin but have undergone distinct molecular differentiation processes, resulting in varying degrees of heterochromatinization. Our data serve as a detailed example of the dynamic and differentiated nature of B chromosome molecular content at the interpopulation level, even when they share a common origin.</p>","PeriodicalId":12809,"journal":{"name":"Genome","volume":" ","pages":"327-338"},"PeriodicalIF":2.3000,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genome","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1139/gen-2023-0122","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/5/9 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Supernumerary chromosomes (B chromosomes) have been an intriguing subject of study. Our understanding of the molecular differentiation of B chromosomes from an interpopulation perspective remains limited, with most analyses involving chromosome banding and mapping of a few sequences. To gain insights into the molecular composition, origin, and evolution of B chromosomes, we conducted cytogenetic and next-generation sequencing analysis of the repeatome in the grasshopper Abracris flavolineata across various populations. Our results unveiled the presence of B chromosomes in two newly investigated populations and described new satellite DNA sequences. While we observed some degree of genetic connection among A. flavolineata populations, our comparative analysis of genomes with and without B chromosomes provided evidence of two new B chromosome variants. These variants exhibited distinct compositions of various repeat classes, including transposable elements and satellite DNAs. Based on shared repeats, their chromosomal location, and the C-positive heterochromatin content on the B chromosome, these variants likely share a common origin but have undergone distinct molecular differentiation processes, resulting in varying degrees of heterochromatinization. Our data serve as a detailed example of the dynamic and differentiated nature of B chromosome molecular content at the interpopulation level, even when they share a common origin.
染色体和基因组分析表明,Abracris flavolineata 的 B 染色体起源单一,分子分化程度较高。
超常染色体(B 染色体)一直是一个引人入胜的研究课题。从种群间的角度来看,我们对 B 染色体分子分化的了解仍然有限,大多数分析都涉及染色体条带和少数序列的图谱绘制。为了深入了解 B 染色体的分子组成、起源和进化,我们对蚱蜢 Abracris flavolineata 不同种群的重复序列组进行了细胞遗传学和新一代测序分析。我们的研究结果揭示了两个新调查种群中 B 染色体的存在,并描述了新的卫星 DNA 序列。虽然我们观察到黄铃虫种群之间存在一定程度的遗传联系,但我们对含有和不含 B 染色体的基因组进行的比较分析提供了两个新的 B 染色体变体的证据。这些变体表现出不同的重复类组成,包括转座元件和卫星 DNA。根据共享的重复序列、它们的染色体位置以及 B 染色体上 C 阳性异染色质的含量,这些变体很可能具有共同的起源,但经历了不同的分子分化过程,导致了不同程度的异染色质化。我们的数据是 B 染色体分子含量在种群间水平的动态和分化性质的一个详细例子,即使它们有共同的起源。
期刊介绍:
Genome is a monthly journal, established in 1959, that publishes original research articles, reviews, mini-reviews, current opinions, and commentaries. Areas of interest include general genetics and genomics, cytogenetics, molecular and evolutionary genetics, developmental genetics, population genetics, phylogenomics, molecular identification, as well as emerging areas such as ecological, comparative, and functional genomics.