D. S. Sitnikov, D. E. Mukhdina, D. S. Korshunova, Yu. Yu. Silaeva, M. A. Filatov, I. V. Ilina
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引用次数: 0
Abstract
The authors studied the dynamics of thinning of the zona pellucida (ZP) in mouse embryo as a result of laser-assisted hatching performed at the blastocyst stage. Mouse embryos previously subjected to a freeze–thaw cycle (cryo-embryos) were selected as model embryos. For ZP microsurgery, femtosecond laser pulses were used (radiation wavelength 512 nm, pulse duration 100 fs, intensity 2.5 TW/cm2). The thickness of the ZP was measured before microsurgery at the blastocyst stage (~E3.5, i.e., 3.5 days of embryonic development) and at the hatching stage (~E5). It was found that the ZP of control group embryos (cryo-embryos not subjected to laser exposure) thinned more strongly (from 6.6 (E3.5) to 4.9 µm (E5)) compared to experimental embryos after the laser-assisted hatching procedure (from 7.1 ( E3.5) to 6.4 µm (E5)). In both the first and second cases, changes in ZP thickness were statistically significant. The results were compared with data for “fresh” embryos not subjected to cryopreservation. There was no pronounced effect of ZP hardening in cryo-embryos compared to fresh embryos. The use of the laser-assisted hatching procedure for cryo-embryos made it possible to increase the probability of successful hatching compared to embryos in the control group from 38.5 to 52.5%.
期刊介绍：
High Temperature is an international peer reviewed journal that publishes original papers and reviews written by theoretical and experimental researchers. The journal deals with properties and processes in low-temperature plasma; thermophysical properties of substances including pure materials, mixtures and alloys; the properties in the vicinity of the critical point, equations of state; phase equilibrium; heat and mass transfer phenomena, in particular, by forced and free convections; processes of boiling and condensation, radiation, and complex heat transfer; experimental methods and apparatuses; high-temperature facilities for power engineering applications, etc. The journal reflects the current trends in thermophysical research. It presents the results of present-day experimental and theoretical studies in the processes of complex heat transfer, thermal, gas dynamic processes, and processes of heat and mass transfer, as well as the latest advances in the theoretical description of the properties of high-temperature media.
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