Screening of indigenous entomopathogenic fungal isolates on plant parasitic nematodes in China

IF 1.7 3区农林科学 Q2 AGRONOMY

European Journal of Plant Pathology Pub Date : 2024-05-03 DOI:10.1007/s10658-024-02870-9

Ming Fang, Jie Sun, Ailing Wang, Hongbo Tang, Lei Wang, Xianqin Wei, Weibin Ruan

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Abstract

Root-knot nematodes (RKNs), including Meloidogyne spp., are renowned as one of the most destructive plant-parasites worldwide. The culture filtrates of entomopathogenic fungi (EPF), obtained through fermentation processes and containing significant enzyme and toxins, exhibit nematicidal activity. Moreover, the host specificity of EPF is notable, with significant variations in host spectrum and virulence among different strains. Thus, screening indigenous EPF against RKNs will broaden the resource base for developing biological control pesticides. In this study, we isolated 25 EPF strains through the Galleria bait method and investigated the pathogenicity against RKNs. Amongst the 25 EPF strains, which were molecularly identified as Beauveria spp., Metarhizium spp., Purpureocillium spp., and Cordyceps spp., several exhibited promising control effects on RKNs. Three EPF culture filters (B. bassiana 25, P. lilacinum 26, and M. pinghaeuse 39) exhibited nematicidal activity that was not affected by illumination methods or temperature. This research indicates the potential of B. bassiana 25, P. lilacinum 26, and M. pinghaeuse 39 as biological agents against RKNs.

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中国本土昆虫病原真菌分离物对植物寄生线虫的影响筛选

根结线虫（RKNs），包括 Meloidogyne spp.，是世界上最具破坏性的植物寄生虫之一。通过发酵过程获得的昆虫病原真菌（EPF）培养滤液含有大量酶和毒素，具有杀线虫活性。此外，EPF 的宿主特异性也很明显，不同菌株的宿主谱和毒力差异很大。因此，筛选针对 RKNs 的本地 EPF 将为开发生物防治农药扩大资源基础。本研究通过 Galleria 诱饵法分离了 25 株 EPF 菌株，并研究了其对 RKNs 的致病性。经分子鉴定，这 25 株 EPF 菌株分别为蒲公英属、元青霉属、紫云英属和冬虫夏草属，其中有几株对 RKNs 具有良好的防治效果。三种 EPF 培养滤器（B. bassiana 25、P. lilacinum 26 和 M. pinghaeuse 39）表现出的杀线虫活性不受光照方法或温度的影响。这项研究表明，B. bassiana 25、P. lilacinum 26 和 M. pinghaeuse 39 具有作为生物制剂防治 RKN 的潜力。

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来源期刊

European Journal of Plant Pathology 农林科学-农艺学

CiteScore

4.20

自引率

5.60%

发文量

183

审稿时长

3 months

期刊介绍： The European Journal of Plant Pathology is an international journal publishing original articles in English dealing with fundamental and applied aspects of plant pathology; considering disease in agricultural and horticultural crops, forestry, and in natural plant populations. The types of articles published are :Original Research at the molecular, physiological, whole-plant and population levels; Mini-reviews on topics which are timely and of global rather than national or regional significance; Short Communications for important research findings that can be presented in an abbreviated format; and Letters-to-the-Editor, where these raise issues related to articles previously published in the journal. Submissions relating to disease vector biology and integrated crop protection are welcome. However, routine screenings of plant protection products, varietal trials for disease resistance, and biological control agents are not published in the journal unless framed in the context of strategic approaches to disease management.