Serum lncRNA RAMP2-AS1 Served as a Biomarker of Deep Vein Thrombosis Occurrence and Development in Elderly

IF 0.9 4区医学

Indian Journal of Hematology and Blood Transfusion Pub Date : 2024-05-06 DOI:10.1007/s12288-024-01782-2

Hailong Zhang, Qichen Feng, Qingfeng Ma, Li Li, Youzhong Xing

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Abstract

The incidence of deep vein thrombosis (DVT) is increasing with aging, which needs a screening and monitoring biomarker. This study focused on the significance of aging- and angiogenesis-related lncRNA RAMP2-AS1 (RAMP2-AS1) aiming to identify a promising biomarker for the incidence of DVT. Serum samples were collected from 63 healthy individuals and 98 patients with DVT. The serum RAMP2-AS1 level was analyzed by PCR and its significance in DVT detection and development prediction was evaluated by ROC and multivariate Cox regression analysis. The regulatory effect of RAMP2-AS1 on endothelial progenitor cells (EPCs) was evaluated by CCK8 and transwell assays. RAMP2-AS1 was significantly downregulated in patients with DVT, which could discriminate patients with DVT from healthy individuals with relatively high sensitivity and specificity. The downregulation of RAMP2-AS1 could predict poor outcomes and was associated with activities of daily living (ADL), neutrophil to lymphocyte ratio (NLR), platelet to lymphocyte ratio (PLR), lymphocyte to monocyte ratio (LMR), and monocyte to high-density lipoprotein cholesterol ratio (MHR) of patients with DVT. RAMP2-AS1 was identified as an independent prognostic factor of DVT by Cox regression analysis. In EPCs, overexpressing RAMP2-AS1 significantly suppressed cell proliferation, migration, and invasion. Downregulated serum RAMP2-AS1 could predict the incidence and progression of DVT. RAMP2-AS1 inhibited EPCs growth and motility, which provides a target for thrombolytic therapy. RAMP2-AS1 level could be included in the risk assessment model of DVT.

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血清lncRNA RAMP2-AS1是老年人深静脉血栓发生和发展的生物标志物

随着年龄的增长，深静脉血栓（DVT）的发病率也在增加，这就需要一种筛选和监测生物标志物。本研究重点研究了与衰老和血管生成相关的lncRNA RAMP2-AS1（RAMP2-AS1）的意义，旨在为深静脉血栓的发病率找出一个有前景的生物标志物。研究人员采集了 63 名健康人和 98 名深静脉血栓患者的血清样本。通过 PCR 分析血清 RAMP2-AS1 水平，并通过 ROC 和多变量 Cox 回归分析评估其在深静脉血栓检测和发展预测中的意义。RAMP2-AS1对内皮祖细胞（EPCs）的调控作用通过CCK8和透孔试验进行了评估。RAMP2-AS1在深静脉血栓患者中明显下调，它能以相对较高的灵敏度和特异性区分深静脉血栓患者和健康人。RAMP2-AS1的下调可预测不良预后，并与深静脉血栓患者的日常生活能力（ADL）、中性粒细胞与淋巴细胞比值（NLR）、血小板与淋巴细胞比值（PLR）、淋巴细胞与单核细胞比值（LMR）和单核细胞与高密度脂蛋白胆固醇比值（MHR）相关。通过 Cox 回归分析，RAMP2-AS1 被确定为深静脉血栓的独立预后因素。在EPCs中，过表达RAMP2-AS1能显著抑制细胞增殖、迁移和侵袭。血清中下调的RAMP2-AS1可预测深静脉血栓的发生和进展。RAMP2-AS1抑制了EPCs的生长和运动，为溶栓治疗提供了靶点。RAMP2-AS1水平可纳入深静脉血栓的风险评估模型。

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来源期刊

Indian Journal of Hematology and Blood Transfusion Medicine-Hematology

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期刊介绍： Indian Journal of Hematology and Blood Transfusion is a medium for propagating and exchanging ideas within the medical community. It publishes peer-reviewed articles on a variety of aspects of clinical hematology, laboratory hematology and hemato-oncology. The journal exists to encourage scientific investigation in the study of blood in health and in disease; to promote and foster the exchange and diffusion of knowledge relating to blood and blood-forming tissues; and to provide a forum for discussion of hematological subjects on a national scale. The Journal is the official publication of The Indian Society of Hematology & Blood Transfusion.