Photovoltaic Tweezers Based on Optical Holography: Application to 2D Trapping of DNA Molecules on a Lithium Niobate Crystal

IF 1 Q4 OPTICS

Optical Memory and Neural Networks Pub Date : 2024-01-30 DOI:10.3103/s1060992x23070214

Lusine Tsarukyan, Anahit Badalyan, Lusine Aloyan, Yeva Dalyan, Rafael Drampyan

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Abstract

The nonuniform 2D photovoltaic fields generated near the surface of a photorefractive Fe-doped lithium niobate (LN:Fe) crystal by a nondiffracting optical Bessel beam with concentric ring structures and 532 nm wavelength are used for the trapping of DNA molecules in NaCl buffer on the crystal surface. The simultaneous observation of the long-living Bessel-like refractive lattice recorded in the LN:Fe crystal and the trapped DNA molecules on the crystal surface was performed by an optical phase microscope operating in the transmission mode. With this approach, the DNA molecules are registered as refractive index nonuniformities on the Bessel lattice refractive index pattern. Observations show that DNA molecules are immobilized and trapped at the borderlines of the concentric rings of the refractive lattice recorded by the Bessel beam. The formation of neutral molecular clusters of DNA by Na⁺ counterions with a nearly globular shape and cluster average size of ~4 μm is revealed. A physical model is developed for the analysis of the electric forces map and explanation of the experimental results. The photovoltaic strategy of trapping and manipulation of micro- and nanoparticles on the crystal surface is promising for the elaboration of the lab-on-a-chip devices operating in an autonomous regime with applications in photonics, micro/nano-electronics and biotechnology.

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基于光学全息技术的光电镊子：应用于 DNA 分子在铌酸锂晶体上的二维捕获

摘要利用波长为 532 nm、具有同心环结构的非折射贝塞尔光束在光折射掺杂铁的铌酸锂 (LN:Fe) 晶体表面附近产生的非均匀二维光电场，在晶体表面捕获 NaCl 缓冲液中的 DNA 分子。利用光学相位显微镜，在透射模式下同时观察 LN:Fe 晶体中记录的长寿命贝塞尔样折射晶格和晶体表面被捕获的 DNA 分子。通过这种方法，DNA 分子在贝塞尔晶格折射率图案上被记录为折射率不均匀。观察结果表明，DNA 分子被固定并被困在贝塞尔光束记录的折射率晶格同心环的边界线上。在 Na+ 反离子的作用下，DNA 形成了中性分子簇，其形状接近球状，簇的平均大小约为 4 μm。为分析电场力图和解释实验结果，建立了一个物理模型。在晶体表面捕获和操纵微米和纳米粒子的光电策略，对于在光子学、微/纳米电子学和生物技术领域应用的自主运行的片上实验室设备的研制是很有前途的。

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来源期刊

Optical Memory and Neural Networks OPTICS-

CiteScore

1.50

自引率

11.10%

发文量

期刊介绍： The journal covers a wide range of issues in information optics such as optical memory, mechanisms for optical data recording and processing, photosensitive materials, optical, optoelectronic and holographic nanostructures, and many other related topics. Papers on memory systems using holographic and biological structures and concepts of brain operation are also included. The journal pays particular attention to research in the field of neural net systems that may lead to a new generation of computional technologies by endowing them with intelligence.