Features of Interaction of Lysozyme with PGLU–PEG Block Copolymers

IF 1.4 4区 化学 Q4 CHEMISTRY, PHYSICAL
L. Yu. Filatova, N. G. Balabushevich
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引用次数: 0

Abstract

The interaction of hen egg white lysozyme and block copolymers of poly(L–glutamic acid sodium salt) and polyethylene glycol (PGLU10–PEG and PGLU100–PEG) have been studied in an aqueous medium and at a water–air interface. Different physicochemical methods have been used: turbidimetry, tensiometry, fluorimetry, circular dichroism spectroscopy, electrophoretic light scattering, and transmission electron microscopy. The formation of mixed adsorption layers at the water–air interface has been observed at block copolymer : enzyme molar ratios of at most 2 : 1. In an aqueous medium, complexation of lysozyme with PGLU–PEG block copolymers leads to the formation of core–shell PGLU10–PEG : lysozyme complexes and PGLU100–PEG : lysozyme complexes with molar compositions of 1 : 1 or 2 : 1. The possibility of regulating the properties of the enzyme–block copolymer interaction products makes it possible to develop strategies for the production of antibacterial drugs.

Abstract Image

Abstract Image

溶菌酶与 PGLU-PEG 嵌段聚合物的相互作用特点
摘要 研究了母鸡蛋清溶菌酶与聚(L-谷氨酸钠盐)和聚乙二醇嵌段共聚物(PGLU10-PEG 和 PGLU100-PEG)在水介质中和水气界面上的相互作用。采用了不同的物理化学方法:浊度法、张力测定法、荧光测定法、圆二色光谱法、电泳光散射法和透射电子显微镜法。在水介质中,溶菌酶与 PGLU-PEG 嵌段共聚物络合后会形成核壳 PGLU10-PEG 溶菌酶复合物和 PGLU100-PEG 溶菌酶复合物,其摩尔比为 1 : 1 或 2 : 1。由于可以调节酶-嵌段共聚物相互作用产物的特性,因此有可能开发出生产抗菌药物的策略。
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来源期刊
Colloid Journal
Colloid Journal 化学-物理化学
CiteScore
2.20
自引率
18.20%
发文量
36
审稿时长
6-12 weeks
期刊介绍: Colloid Journal (Kolloidnyi Zhurnal) is the only journal in Russia that publishes the results of research in the area of chemical science dealing with the disperse state of matter and surface phenomena in disperse systems. The journal covers experimental and theoretical works on a great variety of colloid and surface phenomena: the structure and properties of interfaces; adsorption phenomena and structure of adsorption layers of surfactants; capillary phenomena; wetting films; wetting and spreading; and detergency. The formation of colloid systems, their molecular-kinetic and optical properties, surface forces, interaction of colloidal particles, stabilization, and criteria of stability loss of different disperse systems (lyosols and aerosols, suspensions, emulsions, foams, and micellar systems) are also topics of the journal. Colloid Journal also includes the phenomena of electro- and diffusiophoresis, electro- and thermoosmosis, and capillary and reverse osmosis, i.e., phenomena dealing with the existence of diffusion layers of molecules and ions in the vicinity of the interface.
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