Features of Interaction of Lysozyme with PGLU–PEG Block Copolymers

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
L. Yu. Filatova, N. G. Balabushevich
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引用次数: 0

Abstract

The interaction of hen egg white lysozyme and block copolymers of poly(L–glutamic acid sodium salt) and polyethylene glycol (PGLU10–PEG and PGLU100–PEG) have been studied in an aqueous medium and at a water–air interface. Different physicochemical methods have been used: turbidimetry, tensiometry, fluorimetry, circular dichroism spectroscopy, electrophoretic light scattering, and transmission electron microscopy. The formation of mixed adsorption layers at the water–air interface has been observed at block copolymer : enzyme molar ratios of at most 2 : 1. In an aqueous medium, complexation of lysozyme with PGLU–PEG block copolymers leads to the formation of core–shell PGLU10–PEG : lysozyme complexes and PGLU100–PEG : lysozyme complexes with molar compositions of 1 : 1 or 2 : 1. The possibility of regulating the properties of the enzyme–block copolymer interaction products makes it possible to develop strategies for the production of antibacterial drugs.

Abstract Image

Abstract Image

溶菌酶与 PGLU-PEG 嵌段聚合物的相互作用特点
摘要 研究了母鸡蛋清溶菌酶与聚(L-谷氨酸钠盐)和聚乙二醇嵌段共聚物(PGLU10-PEG 和 PGLU100-PEG)在水介质中和水气界面上的相互作用。采用了不同的物理化学方法:浊度法、张力测定法、荧光测定法、圆二色光谱法、电泳光散射法和透射电子显微镜法。在水介质中,溶菌酶与 PGLU-PEG 嵌段共聚物络合后会形成核壳 PGLU10-PEG 溶菌酶复合物和 PGLU100-PEG 溶菌酶复合物,其摩尔比为 1 : 1 或 2 : 1。由于可以调节酶-嵌段共聚物相互作用产物的特性,因此有可能开发出生产抗菌药物的策略。
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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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