Ectopic expression of an AtBMI1a homolog from Vitis vinifera accelerates flowering time in transgenic Arabidopsis

IF 2.4 3区 农林科学 Q1 Agricultural and Biological Sciences
Jing Feng, Ziyan Lin, Kun Wang, Peipei Wu, Sihong Zhou
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Abstract

BMI1 is the main component of the Polycomb Group Complex 1 (PRC1), which functions as an important epigenetic inhibitor of various regulatory genes associated with shoot apical meristem, embryonic development, flowering, senescence and so on. However, its role in the regulation of grapevine development is still unclear. In order to explore the function of VvBMI1a gene in the regulation of flowering in Vitis vinifera ‘Cabernet Sauvignon’, the subcellular localization, expression pattern, yeast two-hybrid, two-molecule fluorescence complementation and flowering time of transgenic Arabidopsis were analyzed. The results showed that VvBMI1a, a member of RING domain-containing proteins family from grapevine (Vitis vinifera), is involved in controlling plant flowering time. Expression of VvBMI1a was abundant in the shoot tips and inflorescence meristems of grapevine. VvBMI1a protein is localized in the nucleus. VvBMI1a could interact with VvRING1a protein which belonged to PRC1. Phenotypic analysis showed that VvBMI1a over-expression lines had earlier flowering time than wild-type plants under long-day (LD) conditions. Further analysis by qRT-PCR and transcriptome suggests that both the suppression of FLC and activation of FT, SOC1, SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 5 (SPL5), AGL8 were observed in VvBMI1a-overexpressing lines, resulting in early flowering. Our results provide the evidence demonstrating the effectiveness of manipulating VvBMI1a to alter the flowering time of plants. In a word, ectopic overexpression of VvBMI1a inhibited the expression of FLC, activated the expression of FT, SPL5, AGL8 to alter the flowering time of Arabidopsis plants.

Abstract Image

异位表达葡萄中的 AtBMI1a 同源物可加快转基因拟南芥的开花时间
BMI1 是多孔菌群复合体 1(PRC1)的主要成分,是与芽顶端分生组织、胚胎发育、开花、衰老等相关的各种调控基因的重要表观遗传抑制因子。然而,它在葡萄生长发育调控中的作用尚不清楚。为了探索VvBMI1a基因在葡萄'赤霞珠'开花调控中的功能,研究人员分析了VvBMI1a基因的亚细胞定位、表达模式、酵母双杂交、双分子荧光互补以及转基因拟南芥的开花时间。结果表明,葡萄(Vitis vinifera)中的含 RING 结构域蛋白家族成员 VvBMI1a 参与了植物花期的控制。VvBMI1a 在葡萄的芽尖和花序分生组织中大量表达。VvBMI1a 蛋白定位于细胞核中。VvBMI1a 能与属于 PRC1 的 VvRING1a 蛋白相互作用。表型分析表明,在长日照(LD)条件下,VvBMI1a过表达株系的开花时间比野生型植株早。通过 qRT-PCR 和转录组的进一步分析表明,在 VvBMI1a 基因过表达株系中观察到了 FLC 的抑制和 FT、SOC1、SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 5(SPL5)、AGL8 的激活,从而导致提早开花。我们的研究结果提供了证据,证明操纵 VvBMI1a 能有效改变植物的开花时间。总之,异位过表达VvBMI1a抑制了FLC的表达,激活了FT、SPL5和AGL8的表达,从而改变了拟南芥植株的花期。
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来源期刊
Horticulture Environment and Biotechnology
Horticulture Environment and Biotechnology Agricultural and Biological Sciences-Horticulture
CiteScore
4.30
自引率
4.20%
发文量
0
审稿时长
6 months
期刊介绍: Horticulture, Environment, and Biotechnology (HEB) is the official journal of the Korean Society for Horticultural Science, was launched in 1965 as the "Journal of Korean Society for Horticultural Science". HEB is an international journal, published in English, bimonthly on the last day of even number months, and indexed in Biosys Preview, SCIE, and CABI. The journal is devoted for the publication of original research papers and review articles related to vegetables, fruits, ornamental and herbal plants, and covers all aspects of physiology, molecular biology, biotechnology, protected cultivation, postharvest technology, and research in plants related to environment.
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