Jing Feng, Ziyan Lin, Kun Wang, Peipei Wu, Sihong Zhou
{"title":"Ectopic expression of an AtBMI1a homolog from Vitis vinifera accelerates flowering time in transgenic Arabidopsis","authors":"Jing Feng, Ziyan Lin, Kun Wang, Peipei Wu, Sihong Zhou","doi":"10.1007/s13580-024-00600-y","DOIUrl":null,"url":null,"abstract":"<p>BMI1 is the main component of the Polycomb Group Complex 1 (PRC1), which functions as an important epigenetic inhibitor of various regulatory genes associated with shoot apical meristem, embryonic development, flowering, senescence and so on. However, its role in the regulation of grapevine development is still unclear. In order to explore the function of <i>VvBMI1a</i> gene in the regulation of flowering in <i>Vitis vinifera</i> ‘Cabernet Sauvignon’, the subcellular localization, expression pattern, yeast two-hybrid, two-molecule fluorescence complementation and flowering time of transgenic Arabidopsis were analyzed. The results showed that <i>VvBMI1a</i>, a member of RING domain-containing proteins family from grapevine <i>(Vitis vinifera)</i>, is involved in controlling plant flowering time. Expression of <i>VvBMI1a</i> was abundant in the shoot tips and inflorescence meristems of grapevine. VvBMI1a protein is localized in the nucleus. VvBMI1a could interact with VvRING1a protein which belonged to PRC1. Phenotypic analysis showed that <i>VvBMI1a</i> over-expression lines had earlier flowering time than wild-type plants under long-day (LD) conditions. Further analysis by qRT-PCR and transcriptome suggests that both the suppression of <i>FLC</i> and activation of <i>FT</i>, <i>SOC1</i>, <i>SQUAMOSA PROMOTER BINDING PROTEIN-LIKE</i> 5 (<i>SPL5</i>), <i>AGL8</i> were observed in <i>VvBMI1a</i>-overexpressing lines, resulting in early flowering. Our results provide the evidence demonstrating the effectiveness of manipulating <i>VvBMI1a</i> to alter the flowering time of plants. In a word, ectopic overexpression of <i>VvBMI1a</i> inhibited the expression of <i>FLC</i>, activated the expression of <i>FT</i>, <i>SPL5</i>, <i>AGL8</i> to alter the flowering time of <i>Arabidopsis</i> plants.</p>","PeriodicalId":13123,"journal":{"name":"Horticulture Environment and Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.4000,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Horticulture Environment and Biotechnology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s13580-024-00600-y","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 0
Abstract
BMI1 is the main component of the Polycomb Group Complex 1 (PRC1), which functions as an important epigenetic inhibitor of various regulatory genes associated with shoot apical meristem, embryonic development, flowering, senescence and so on. However, its role in the regulation of grapevine development is still unclear. In order to explore the function of VvBMI1a gene in the regulation of flowering in Vitis vinifera ‘Cabernet Sauvignon’, the subcellular localization, expression pattern, yeast two-hybrid, two-molecule fluorescence complementation and flowering time of transgenic Arabidopsis were analyzed. The results showed that VvBMI1a, a member of RING domain-containing proteins family from grapevine (Vitis vinifera), is involved in controlling plant flowering time. Expression of VvBMI1a was abundant in the shoot tips and inflorescence meristems of grapevine. VvBMI1a protein is localized in the nucleus. VvBMI1a could interact with VvRING1a protein which belonged to PRC1. Phenotypic analysis showed that VvBMI1a over-expression lines had earlier flowering time than wild-type plants under long-day (LD) conditions. Further analysis by qRT-PCR and transcriptome suggests that both the suppression of FLC and activation of FT, SOC1, SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 5 (SPL5), AGL8 were observed in VvBMI1a-overexpressing lines, resulting in early flowering. Our results provide the evidence demonstrating the effectiveness of manipulating VvBMI1a to alter the flowering time of plants. In a word, ectopic overexpression of VvBMI1a inhibited the expression of FLC, activated the expression of FT, SPL5, AGL8 to alter the flowering time of Arabidopsis plants.
期刊介绍:
Horticulture, Environment, and Biotechnology (HEB) is the official journal of the Korean Society for Horticultural Science, was launched in 1965 as the "Journal of Korean Society for Horticultural Science".
HEB is an international journal, published in English, bimonthly on the last day of even number months, and indexed in Biosys Preview, SCIE, and CABI.
The journal is devoted for the publication of original research papers and review articles related to vegetables, fruits, ornamental and herbal plants, and covers all aspects of physiology, molecular biology, biotechnology, protected cultivation, postharvest technology, and research in plants related to environment.