FISH and GISH reveal genome composition of popular Narcissus cultivars and the possible ways of their origin

IF 1.6 3区农林科学 Q2 AGRONOMY

Euphytica Pub Date : 2024-05-06 DOI:10.1007/s10681-024-03350-1

Yanni Sun, Jie Zeng, Siyu Liu, Shujun Zhou

{"title":"FISH and GISH reveal genome composition of popular Narcissus cultivars and the possible ways of their origin","authors":"Yanni Sun, Jie Zeng, Siyu Liu, Shujun Zhou","doi":"10.1007/s10681-024-03350-1","DOIUrl":null,"url":null,"abstract":"There are thousands of Narcissus cultivars, however; their genome compositions are not so clear, except that their ploidy levels are extensively investigated. The present study analyzed eight popular cultivars using GISH or/and FISH. The present results clearly revealed their genome compositions: ‘Bridal Crown’ was diploid (2n = 2x = 17 = 10 + 7 = AE) rather than aneuploid, ‘Jinzhanyintai’ was autotriploid (2n = 3x = 30 = 10 + 10 + 10 = AAA) rather than allotetraploid or hexaploid, ‘Tête-à-Tête’ was allotriploid (2n = 3x = 24 + B = 10 + 7 + 7 + B = AEE), ‘Cultivar X’ was also allotriploid (2n = 3x = 24 = 10 + 7 + 7 = AEX), and ‘Dutch Master’, ‘Replete’ and ‘Queen’s Day’ were all autotetraploid (2n = 4x = 28 = 7 + 7 + 7 + 7 = EEEE). Together with other reports, we concluded that ‘Tête-à-Tête’ contained one genome of Narcissus tazetta, one genome of Narcissus cyclamineus, and one genome of Narcissus pseudonarcissus, and that ‘Pink Parasol’ was aneu-autotetraploid (2n = 4x + 2 = 30 = 7 + 7 + 7 + 7 + 2 = EEEE+2) rather allotetraploid (2n = 4x = 10 + 10 + 5 + 5 = CCDD). Based on the cultivar’s genome composition and origin, it is concluded that distant hybridization, sexual polyploidization and chromosome doubling play different roles in breeding modern Narcissus cultivars.","PeriodicalId":11803,"journal":{"name":"Euphytica","volume":"101 1","pages":""},"PeriodicalIF":1.6000,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Euphytica","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s10681-024-03350-1","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRONOMY","Score":null,"Total":0}

引用次数: 0

Abstract

There are thousands of Narcissus cultivars, however; their genome compositions are not so clear, except that their ploidy levels are extensively investigated. The present study analyzed eight popular cultivars using GISH or/and FISH. The present results clearly revealed their genome compositions: ‘Bridal Crown’ was diploid (2n = 2x = 17 = 10 + 7 = AE) rather than aneuploid, ‘Jinzhanyintai’ was autotriploid (2n = 3x = 30 = 10 + 10 + 10 = AAA) rather than allotetraploid or hexaploid, ‘Tête-à-Tête’ was allotriploid (2n = 3x = 24 + _B = 10 + 7 + 7 + _B = AEE), ‘Cultivar X’ was also allotriploid (2n = 3x = 24 = 10 + 7 + 7 = AEX), and ‘Dutch Master’, ‘Replete’ and ‘Queen’s Day’ were all autotetraploid (2n = 4x = 28 = 7 + 7 + 7 + 7 = EEEE). Together with other reports, we concluded that ‘Tête-à-Tête’ contained one genome of Narcissus tazetta, one genome of Narcissus cyclamineus, and one genome of Narcissus pseudonarcissus, and that ‘Pink Parasol’ was aneu-autotetraploid (2n = 4x + 2 = 30 = 7 + 7 + 7 + 7 + 2 = EEEE⁺²) rather allotetraploid (2n = 4x = 10 + 10 + 5 + 5 = CCDD). Based on the cultivar’s genome composition and origin, it is concluded that distant hybridization, sexual polyploidization and chromosome doubling play different roles in breeding modern Narcissus cultivars.

Abstract Image

查看原文本刊更多论文

FISH 和 GISH 揭示流行水仙栽培品种的基因组组成及其可能的起源方式

水仙栽培品种数以千计，但除了对其倍性水平进行广泛研究外，其基因组组成并不十分清楚。本研究利用 GISH 或/和 FISH 分析了 8 个流行的栽培品种。研究结果清楚地揭示了它们的基因组组成：新娘皇冠'是二倍体（2n = 2x = 17 = 10 + 7 = AE）而不是非整倍体，'金盏银台'是自三倍体（2n = 3x = 30 = 10 + 10 + 10 = AAA）而不是异源四倍体或六倍体，'Tête-à-Tête'是异源三倍体（2n = 3x = 24 + B = 10 + 7 + 7 + B = AEE）、栽培品种 X'也是异源三倍体（2n = 3x = 24 = 10 + 7 + 7 = AEX），而'荷兰大师'、'Replete'和'女王日'都是自交四倍体（2n = 4x = 28 = 7 + 7 + 7 + 7 = EEEE）。结合其他报告，我们得出结论，'Tête-à-Tête'含有一个水仙（Narcissus tazetta）基因组、一个水仙（Narcissus cyclamineus）基因组和一个水仙（Narcissus pseudonarcissus）基因组，而'Pink Parasol'是一个自交四倍体（2n = 4x + 2 = 30 = 7 + 7 + 7 + 7 + 2 = EEEE+2），而不是异源四倍体（2n = 4x = 10 + 10 + 5 + 5 = CCDD）。根据栽培品种的基因组组成和起源，可以得出结论：远缘杂交、有性多倍体化和染色体加倍在培育现代水仙栽培品种中发挥着不同的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Euphytica 农林科学-农艺学

CiteScore

3.80

自引率

5.30%

发文量

157

审稿时长

4.5 months

期刊介绍： Euphytica is an international journal on theoretical and applied aspects of plant breeding. It publishes critical reviews and papers on the results of original research related to plant breeding. The integration of modern and traditional plant breeding is a growing field of research using transgenic crop plants and/or marker assisted breeding in combination with traditional breeding tools. The content should cover the interests of researchers directly or indirectly involved in plant breeding, at universities, breeding institutes, seed industries, plant biotech companies and industries using plant raw materials, and promote stability, adaptability and sustainability in agriculture and agro-industries.