Enhanced Aerobic Naphthalene Degradation Utilizing Indigenous Microbial Flora as a Biocatalyst in Oil-Contaminated Wastewater

IF 2.8 3区 化学 Q2 CHEMISTRY, APPLIED
Ponnuswamy Vijayaraghavan, Veeramani Veeramanikandan, Bhathini Vaikuntavasan Pradeep, Chinnathambi Pothiraj, Khaloud Mohammed Alarjani, Dunia A. Al Farraj, Van-Huy Nguyen, Paulraj Balaji
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Abstract

Bacteria indigenous to oil-contaminated water exhibited diverse metabolic capabilities in degrading various aromatic and monoaromatic hydrocarbons. Out of the 28 bacterial strains isolated from the wastewater, each was cultivated with at least one hydrocarbon, including kerosene, naphthalene, toluene, diesel, or aniline. Among these strains, Pseudomonas putida AD-128 emerged as one of the most effective polyaromatic hydrocarbon (PAH) degraders. Following a 6-day treatment period, strain P. putida AD-128 demonstrated proficiency in degrading various PAHs, including naphthalene, phenanthrene, and fluorine. After 6 days of incubation at 20 °C, the degradation of Naphthalene (NAP) notably increased. Gas Chromatography Mass Spectrometry analysis identified the degraded compounds, including pyruvic acid, salicylaldehyde, D-gluconic acid, and catechol. Optimal NAP degradation was observed at 20 °C and pH 6.0, with increased agitation speed correlating with enhanced bacterial growth and heightened degradation, particularly evident after 6 days at 20 °C. Peptone emerged as the most effective among the four nitrogen supplements (ammonium sulfate, potassium nitrate, beef extract, and peptone), significantly reducing residual naphthalene in the medium. The isolated indigenous bacterium, P. putida AD-128, exhibits robust capabilities in degrading PAHs under optimized conditions, making it a valuable asset for environmental management initiatives.

Abstract Image

在受石油污染的废水中利用本地微生物菌群作为生物催化剂增强需氧萘降解能力
石油污染水体中的本地细菌在降解各种芳香族和单芳香族碳氢化合物方面表现出不同的代谢能力。在从废水中分离出的 28 株细菌中,每株都至少用一种碳氢化合物进行过培养,包括煤油、萘、甲苯、柴油或苯胺。在这些菌株中,假单胞菌 AD-128 是最有效的多芳烃(PAH)降解菌之一。经过 6 天的处理,P. putida AD-128 菌株表现出了降解各种 PAHs(包括萘、菲和氟)的能力。在 20 °C 下培养 6 天后,萘(NAP)的降解量明显增加。气相色谱质谱分析确定了降解的化合物,包括丙酮酸、水杨醛、D-葡萄糖酸和邻苯二酚。在 20 °C、pH 值为 6.0 的条件下,NAP 降解效果最佳,搅拌速度的提高与细菌生长的增强和降解的加强相关,尤其是在 20 °C条件下 6 天后更为明显。在四种氮补充剂(硫酸铵、硝酸钾、牛肉提取物和蛋白胨)中,蛋白胨最为有效,可显著减少培养基中的残留萘。分离出的本地细菌 P. putida AD-128 在优化条件下表现出降解多环芳烃的强大能力,使其成为环境管理措施的宝贵资产。
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来源期刊
Topics in Catalysis
Topics in Catalysis 化学-物理化学
CiteScore
5.70
自引率
5.60%
发文量
197
审稿时长
2 months
期刊介绍: Topics in Catalysis publishes topical collections in all fields of catalysis which are composed only of invited articles from leading authors. The journal documents today’s emerging and critical trends in all branches of catalysis. Each themed issue is organized by renowned Guest Editors in collaboration with the Editors-in-Chief. Proposals for new topics are welcome and should be submitted directly to the Editors-in-Chief. The publication of individual uninvited original research articles can be sent to our sister journal Catalysis Letters. This journal aims for rapid publication of high-impact original research articles in all fields of both applied and theoretical catalysis, including heterogeneous, homogeneous and biocatalysis.
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