miR-34b-5p suppresses the epithelial-mesenchymal transition and metastasis in endometrial cancer AN3CA cells by targeting ZEB1.

IF 1.1 Q4 ONCOLOGY
International journal of clinical and experimental pathology Pub Date : 2024-04-15 eCollection Date: 2024-01-01 DOI:10.62347/JVBV7887
Lulu Shi, Dan Yang, Hui Dong, Xueyu Zhang, Caihong Yang
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引用次数: 0

Abstract

Objectives: Tumor metastasis is a primary cause of recurrence and mortality in endometrial cancer. miR-34b-5p is abnormally expressed in various cancers and participates in tumor cell progression and metastasis. The objective of this study was to elucidate the biological functions and molecular mechanisms of miR-34b-5p in regulating the epithelial-mesenchymal transition (EMT) and metastasis in AN3CA endometrial cancer cells.

Methods: The expression levels of miR-34b-5p and zinc finger E-box-binding homeobox 1 (ZEB1) in endometrial cancer cells were analyzed by qRT-PCR, and ZEB1 expression in endometrial cancer tissues was examined by immunohistochemistry. Proliferation, migration, and invasion of endometrial cancer AN3CA cells were evaluated using CCK8, scratch, and transwell assays, respectively. Bioinformatic analysis and dual-luciferase reporter gene assays were used to validate the targeting relationship between miR-34b-5p and ZEB1. Western blotting was performed to analyze the expression levels of ZEB1 and EMT-related proteins.

Results: miR-34b-5p was significantly downregulated in endometrial cancer AN3CA cells. Overexpression of miR-34b-5p significantly inhibited proliferation, invasion, migration, and the EMT of endometrial cancer AN3CA cells. ZEB1, which was identified as a direct target gene of miR-34b-5p, exhibited high expression in endometrial cancer cells and tissues. Additionally, ZEB1 upregulation partially reversed the inhibitory effects of miR-34b-5p on proliferation, migration, invasion, and the EMT of endometrial cancer AN3CA cells.

Conclusions: miR-34b-5p suppresses the EMT and metastasis in endometrial cancer AN3CA cells by targeting ZEB1, indicating that the miR-34b-5p-ZEB1-EMT axis may be a therapeutic target for endometrial cancer.

miR-34b-5p 通过靶向 ZEB1 抑制子宫内膜癌 AN3CA 细胞的上皮-间质转化和转移。
目的:肿瘤转移是子宫内膜癌复发和死亡的主要原因:miR-34b-5p在多种癌症中异常表达,并参与肿瘤细胞的进展和转移。本研究旨在阐明miR-34b-5p调控AN3CA子宫内膜癌细胞上皮-间质转化(EMT)和转移的生物学功能和分子机制:方法:采用qRT-PCR方法分析miR-34b-5p和锌指E-盒结合同工酶1(ZEB1)在子宫内膜癌细胞中的表达水平,并采用免疫组化方法检测ZEB1在子宫内膜癌组织中的表达。子宫内膜癌 AN3CA 细胞的增殖、迁移和侵袭分别通过 CCK8、划痕和跨孔试验进行了评估。生物信息学分析和双荧光素酶报告基因实验验证了 miR-34b-5p 和 ZEB1 之间的靶向关系。结果:miR-34b-5p在子宫内膜癌AN3CA细胞中显著下调。过表达 miR-34b-5p 能明显抑制子宫内膜癌 AN3CA 细胞的增殖、侵袭、迁移和 EMT。ZEB1是miR-34b-5p的直接靶基因,在子宫内膜癌细胞和组织中表现出高表达。结论:miR-34b-5p通过靶向ZEB1抑制子宫内膜癌AN3CA细胞的EMT和转移,表明miR-34b-5p-ZEB1-EMT轴可能是子宫内膜癌的治疗靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
自引率
0.00%
发文量
42
审稿时长
1 months
期刊介绍: The International Journal of Clinical and Experimental Pathology (IJCEP, ISSN 1936-2625) is a peer reviewed, open access online journal. It was founded in 2008 by an international group of academic pathologists and scientists who are devoted to the scientific exploration of human disease and the rapid dissemination of original data. Unlike most other open access online journals, IJCEP will keep all the traditional features of paper print that we are all familiar with, such as continuous volume and issue numbers, as well as continuous page numbers to keep our warm feelings towards an academic journal. Unlike most other open access online journals, IJCEP will keep all the traditional features of paper print that we are all familiar with, such as continuous volume and issue numbers, as well as continuous page numbers to keep our warm feelings towards an academic journal.
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