Diversity and Functionality of Bacteria Associated with Different Tissues of Spider Heteropoda venatoria Revealed through Integration of High-Throughput Sequencing and Culturomics Approaches.

IF 3.3 3区 生物学 Q2 ECOLOGY
Likun Zhao, Shanfeng Zhang, Ruoyi Xiao, Chao Zhang, Zhitang Lyu, Feng Zhang
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Abstract

Spiders host a diverse range of bacteria in their guts and other tissues, which have been found to play a significant role in their fitness. This study aimed to investigate the community diversity and functional characteristics of spider-associated bacteria in four tissues of Heteropoda venatoria using HTS of the 16S rRNA gene and culturomics technologies, as well as the functional verification of the isolated strains. The results of HTS showed that the spider-associated bacteria in different tissues belonged to 34 phyla, 72 classes, 170 orders, 277 families, and 458 genera. Bacillus was found to be the most abundant bacteria in the venom gland, silk gland, and ovary, while Stenotrophomonas, Acinetobacter, and Sphingomonas were dominant in the gut microbiota. Based on the amplicon sequencing results, 21 distinct cultivation conditions were developed using culturomics to isolate bacteria from the ovary, gut, venom gland, and silk gland. A total of 119 bacterial strains, representing 4 phyla and 25 genera, with Bacillus and Serratia as the dominant genera, were isolated. Five strains exhibited high efficiency in degrading pesticides in the in vitro experiments. Out of the 119 isolates, 28 exhibited antibacterial activity against at least one of the tested bacterial strains, including the pathogenic bacteria Staphylococcus aureus, Acinetobacter baumanii, and Enterococcus faecalis. The study also identified three strains, GL312, PL211, and PL316, which exhibited significant cytotoxicity against MGC-803. The crude extract from the fermentation broth of strain PL316 was found to effectively induce apoptosis in MGC-803 cells. Overall, this study offers a comprehensive understanding of the bacterial community structure associated with H. venatoria. It also provides valuable insights into discovering novel antitumor natural products for gastric cancer and xenobiotic-degrading bacteria of spiders.

Abstract Image

通过整合高通量测序和培养组学方法揭示蜘蛛 Heteropoda venatoria 不同组织相关细菌的多样性和功能性
蜘蛛的内脏和其他组织中寄生着多种多样的细菌,这些细菌对蜘蛛的生存能力起着重要作用。本研究旨在利用 16S rRNA 基因的 HTS 和培养组学技术,研究蜘蛛四种组织中蜘蛛相关细菌的群落多样性和功能特征,并对分离菌株进行功能验证。HTS结果显示,不同组织中的蜘蛛相关细菌隶属于34门、72纲、170目、277科、458属。在毒腺、丝腺和卵巢中,芽孢杆菌的数量最多,而在肠道微生物群中,则以臭单胞菌、醋杆菌和鞘氨单胞菌为主。根据扩增子测序结果,利用培养组学开发了21种不同的培养条件,从卵巢、肠道、毒腺和丝腺分离细菌。共分离出 119 株细菌,代表 4 个门 25 个属,其中芽孢杆菌和沙雷氏菌是优势菌属。在体外实验中,有 5 株菌株表现出较高的农药降解效率。在 119 株分离菌株中,28 株对至少一种受测细菌菌株具有抗菌活性,包括金黄色葡萄球菌、鲍曼不动杆菌和粪肠球菌等致病菌。研究还发现 GL312、PL211 和 PL316 三种菌株对 MGC-803 具有显著的细胞毒性。研究发现,从菌株 PL316 的发酵液中提取的粗提取物能有效诱导 MGC-803 细胞凋亡。总之,本研究全面了解了与 H. venatoria 相关的细菌群落结构。它还为发现新型胃癌抗肿瘤天然产物和蜘蛛的异生物降解细菌提供了宝贵的见解。
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来源期刊
Microbial Ecology
Microbial Ecology 生物-海洋与淡水生物学
CiteScore
6.90
自引率
2.80%
发文量
212
审稿时长
3-8 weeks
期刊介绍: The journal Microbial Ecology was founded more than 50 years ago by Dr. Ralph Mitchell, Gordon McKay Professor of Applied Biology at Harvard University in Cambridge, MA. The journal has evolved to become a premier location for the presentation of manuscripts that represent advances in the field of microbial ecology. The journal has become a dedicated international forum for the presentation of high-quality scientific investigations of how microorganisms interact with their environment, with each other and with their hosts. Microbial Ecology offers articles of original research in full paper and note formats, as well as brief reviews and topical position papers.
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