In silico identification and expression analysis of superoxide dismutases in Tenebrio molitor.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-07-01 Epub Date: 2024-05-03 DOI:10.1007/s13258-024-01518-6

Ho Am Jang, Hyeonjun Shin, Seo Jin Lee, Sung Min Ku, Jae Hui Kim, Dong Woo Kang, So Yeon Choi, Sang Mok Jung, Hyun Woung Shin, Yong Seok Lee, Yeon Soo Han, Yong Hun Jo

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引用次数: 0

Abstract

Background: Insects encounter various environmental stresses, in response to which they generate reactive oxygen species (ROS). Superoxide dismutase (SOD) is an antioxidant metalloenzyme that scavenges superoxide radicals to prevent oxidative damage.

Objective: To investigate expressions of SODs under oxidative stress in Tenebrio molitor.

Methods: Here, we investigated the transcriptional expression of SODs by pesticide and heavy metals in Tenebrio moltior. First, we searched an RNA-Seq database for T. molitor SOD (TmSOD) genes and identified two SOD isoforms (TmSOD1-iso1 and iso2). We examined their activities under developmental stage, tissue-specific, and various types (pesticide and heavy metal) of oxidative stress by using qPCR.

Results: Our results revealed two novel forms of TmSODs. These TmSODs had a copper/zinc superoxide dismutase domain, active site, Cu²⁺ binding site, Zn²⁺ binding site, E-class dimer interface, and P-class dimer interface. TmSODs (TmSOD1-iso1 and iso2) were expressed in diverse developmental phases and tissues. Pesticides and heavy metals caused an upregulation of these TmSODs.

Conclusion: Our findings suggest that the two TmSODs have different functions in T. molitor, providing insights into the detoxification ability of T. molitor.

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超氧化物歧化酶的硅学鉴定和表达分析。

背景：昆虫会遇到各种环境压力，从而产生活性氧（ROS）。超氧化物歧化酶（SOD）是一种抗氧化金属酶，可清除超氧自由基，防止氧化损伤：方法：我们研究了农药和重金属对褐天牛体内 SODs 的转录表达。首先，我们在 RNA-Seq 数据库中搜索了褐飞虱 SOD（TmSOD）基因，并确定了两种 SOD 异构体（TmSOD1-iso1 和 iso2）。我们使用 qPCR 方法检测了它们在发育阶段、特定组织和各种类型（农药和重金属）氧化应激下的活性：结果：我们发现了两种新形式的 TmSODs。这些TmSODs具有铜/锌超氧化物歧化酶结构域、活性位点、Cu2+结合位点、Zn2+结合位点、E级二聚体界面和P级二聚体界面。TmSOD（TmSOD1-iso1和iso2）在不同的发育阶段和组织中表达。农药和重金属导致这些 TmSODs 上调：我们的研究结果表明，两种 TmSOD 在褐飞虱体内具有不同的功能，为了解褐飞虱的解毒能力提供了线索。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.