Electrophysiological and morphological properties of prefrontal pyramidal neurons innervated by mediodorsal thalamus.

Q3 Medicine
生理学报 Pub Date : 2024-04-25
Zu-Quan Fan, Xiao-Dong Tao, Ya-Ru Wei, Xue-Han Zhang
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引用次数: 0

Abstract

The high-order cognitive and executive functions are necessary for an individual to survive. The densely bidirectional innervations between the medial prefrontal cortex (mPFC) and the mediodorsal thalamus (MD) play a vital role in regulating high-order functions. Pyramidal neurons in mPFC have been classified into several subclasses according to their morphological and electrophysiological properties, but the properties of the input-specific pyramidal neurons in mPFC remain poorly understood. The present study aimed to profile the morphological and electrophysiological properties of mPFC pyramidal neurons innervated by MD. In the past, the studies for characterizing the morphological and electrophysiological properties of neurons mainly relied on the electrophysiological recording of a large number of neurons and their morphologic reconstructions. But, it is a low efficient method for characterizing the circuit-specific neurons. The present study combined the advantages of traditional morphological and electrophysiological methods with machine learning to address the shortcomings of the past method, to establish a classification model for the morphological and electrophysiological properties of mPFC pyramidal neurons, and to achieve more accurate and efficient identification of the properties from a small size sample of neurons. We labeled MD-innervated pyramidal neurons of mPFC using the trans-synaptic neural circuitry tracing method and obtained their morphological properties using whole-cell patch-clamp recording and morphologic reconstructions. The results showed that the classification model established in the present study could predict the electrophysiological properties of MD-innervated pyramidal neurons based on their morphology. MD-innervated pyramidal neurons exhibit larger basal dendritic length but lower apical dendrite complexity compared to non-MD-innervated neurons in the mPFC. The morphological characteristics of the two subtypes (ET-1 and ET-2) of mPFC pyramidal neurons innervated by MD are different, with the apical dendrites of ET-1 neurons being longer and more complex than those of ET-2 neurons. These results suggest that the electrophysiological properties of MD- innervated pyramidal neurons within mPFC correlate with their morphological properties, indicating that the different roles of these two subclasses in local circuits within PFC, as well as in PFC-cortical/subcortical brain region circuits.

丘脑内侧支配的前额叶锥体神经元的电生理学和形态学特性
高阶认知和执行功能是个体生存的必要条件。内侧前额叶皮层(mPFC)和丘脑内侧之间密集的双向神经支配在调节高阶功能方面发挥着至关重要的作用。mPFC中的锥体神经元已根据其形态学和电生理学特性被分为多个亚类,但对mPFC中输入特异性锥体神经元的特性仍知之甚少。本研究的目的是分析由MD支配的mPFC锥体神经元的形态和电生理特性。过去,神经元形态和电生理特性的研究主要依赖于大量神经元的电生理记录及其形态重建。但是,这种方法表征特定回路神经元的效率较低。本研究将传统形态学和电生理学方法的优点与机器学习相结合,解决了以往方法的不足,建立了 mPFC 锥体神经元形态学和电生理学特性的分类模型,实现了从小规模神经元样本中更准确、更高效地识别其特性。我们采用跨突触神经回路追踪方法标记了mPFC受MD神经支配的锥体神经元,并通过全细胞贴片钳记录和形态重建获得了它们的形态学特性。结果表明,本研究建立的分类模型可以根据MD神经支配锥体神经元的形态预测其电生理特性。与 mPFC 中未受 MD 神经元支配的神经元相比,受 MD 神经元支配的锥体神经元表现出更大的基底树突长度,但顶端树突复杂性较低。由 MD 神经元支配的 mPFC 锥体神经元的两个亚型(ET-1 和 ET-2)的形态特征不同,ET-1 神经元的顶端树突比 ET-2 神经元的树突更长、更复杂。这些结果表明,mPFC 中受 MD 神经元支配的锥体神经元的电生理特性与其形态学特性相关,表明这两个亚类神经元在 PFC 的局部回路以及 PFC-皮层/皮层下脑区回路中发挥着不同的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
生理学报
生理学报 Medicine-Medicine (all)
CiteScore
1.20
自引率
0.00%
发文量
4820
期刊介绍: Acta Physiologica Sinica (APS) is sponsored by the Chinese Association for Physiological Sciences and Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences (CAS), and is published bimonthly by the Science Press, China. APS publishes original research articles in the field of physiology as well as research contributions from other biomedical disciplines and proceedings of conferences and symposia of physiological sciences. Besides “Original Research Articles”, the journal also provides columns as “Brief Review”, “Rapid Communication”, “Experimental Technique”, and “Letter to the Editor”. Articles are published in either Chinese or English according to authors’ submission.
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