Short term biodistribution and in vivo toxicity assessment of intravenously injected pristine graphene oxide nanoflakes in SD rats.

IF 2.2 4区 医学 Q3 TOXICOLOGY
Toxicology Research Pub Date : 2024-04-12 eCollection Date: 2024-04-01 DOI:10.1093/toxres/tfae058
Indranil De, Rashmika Singh, Sushil Kumar, Srishti Singh, Manohar Singh, Jiban Jyoti Panda, Kaushik Ghosh, Durga Prasad Mishra, Manish Singh
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Abstract

The present study aimed to elucidate the short term biodistribution of nano sized graphene oxide (GO) along with the toxicological assessment under in-vivo condition with an intent to analyse the toxic effects of sudden accidental exposure of GO The synthesised GO was characterized using UV-Visible spectroscopy, XRD, FTIR, Raman spectroscopy, TGA and DLS. The morphological imaging was performed using SEM, TEM and AFM. With a lateral size of less than 300 nm, these nanoparticles exhibit significant organ barrier permeability of up to 20%. Upon acute exposure to 10 mg/kg dose of ICG-tagged GO nanoflakes through intravenous route, various organs such as kidney, spleen and liver were observed, and the nanoparticles predominantly accumulated in the liver upon 24 h of exposure. Upon confirming the accumulation of these particles in liver through IVIS imaging, our next attempt was to analyse various biochemical and serum parameters. An elevation in various serum parameters such as ALT, AST, Creatinine and Bilirubin was observed. Similarly, in the case of biochemical parameters tested in liver homogenates, an increase in NO, Catalase, GSH, SOD, ROS, LPO, GR, GPx, and GST was observed. This study highlights the potential toxicological risk associated with GO exposure which must be taken into account for any risk analysis associated with GO based consumer products and the occupational hazards.

在 SD 大鼠体内静脉注射原始氧化石墨烯纳米片的短期生物分布和体内毒性评估。
本研究旨在阐明纳米级氧化石墨烯(GO)的短期生物分布以及体内毒理学评估,以分析突然意外接触 GO 的毒性效应。使用扫描电子显微镜(SEM)、电子显微镜(TEM)和原子力显微镜(AFM)进行了形态成像。这些纳米颗粒的横向尺寸小于 300 纳米,具有显著的器官屏障渗透性,渗透率高达 20%。通过静脉途径急性暴露于 10 mg/kg 剂量的 ICG 标记 GO 纳米片后,可观察到肾脏、脾脏和肝脏等多个器官,暴露 24 小时后,纳米颗粒主要在肝脏中蓄积。在通过 IVIS 成像确认这些颗粒在肝脏中聚集后,我们下一步尝试分析各种生化和血清参数。观察到 ALT、AST、肌酐和胆红素等各种血清参数升高。同样,在检测肝匀浆中的生化参数时,也观察到 NO、过氧化氢酶、GSH、SOD、ROS、LPO、GR、GPx 和 GST 的增加。这项研究强调了与接触 GO 有关的潜在毒理学风险,在对基于 GO 的消费品和职业危害进行风险分析时,必须考虑到这一点。
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来源期刊
Toxicology Research
Toxicology Research TOXICOLOGY-
CiteScore
3.60
自引率
0.00%
发文量
82
期刊介绍: A multi-disciplinary journal covering the best research in both fundamental and applied aspects of toxicology
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