A comparative study of the in vitro dermal absorption of radiolabeled benzophenone through human skin

IF 2.6 3区医学 Q3 TOXICOLOGY

Toxicology in Vitro Pub Date : 2024-04-26 DOI:10.1016/j.tiv.2024.105835

Sadaff Ejaz , Clive Roper , Zoe Finlayson , Kyle S. Saitta , Timothy McCarthy , Frank Sun , Michael D. Southall

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引用次数: 0

Abstract

Octocrylene is a common sun filter ingredient used to protect the skin from damaging UV rays. Benzophenone is an impurity found in formulations containing octocrylene. [¹⁴C]-Benzophenone was spiked (0.1 g/L) into 2 commercial sunscreen formulations; Neutrogena® Beach Defense Sunscreen Spray Broad Spectrum SPF 70 Aerosol, Neutrogena® Ultra Sheer Body Mist Sunscreen Broad Spectrum SPF 30 Aerosol, and an acetone vehicle. The formulations were applied (ca 2 μL/cm²) to dermatomed human skin mounted in static diffusion cells in vitro. Receptor fluid was collected up to 24 h post dose. All samples were analyzed by liquid scintillation counting. The dermal delivery of [¹⁴C]-Benzophenone was 10.02, 9.04 and 5.19% for the 3 formulations. However, the [¹⁴C]-Benzophenone mass balances were low; 81.5, 85.3 and 8.02%, respectively. A volatility test was performed replacing skin with aluminum foil for the sunscreen formulations only. The [¹⁴C]-Benzophenone mass balance at dosing was 99% but fell to 56.9 and 60.6% at 24 h post dose, confirming the losses were due to [¹⁴C]-Benzophenone volatility. A conservative dermal absorption value of 12.42% was proposed to cover [¹⁴C]-Benzophenone containing formulations.

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放射性标记二苯甲酮通过人体皮肤的体外皮肤吸收比较研究

辛二烯是一种常见的防晒成分，用于保护皮肤免受紫外线的伤害。二苯甲酮是含有辛丙烯的配方中的一种杂质。将 [14C]-Benzophenone 添加（0.1 g/L）到两种商用防晒霜配方中：露得清®海滩防晒喷雾广谱 SPF 70 气雾剂、露得清®超薄身体喷雾防晒霜广谱 SPF 30 气雾剂和丙酮载体。将这些配方（约 2 μL/cm2）涂抹到安装在体外静态扩散细胞中的皮肤上。在用药后 24 小时内收集受体液。所有样本均采用液体闪烁计数法进行分析。三种配方的[14C]-二苯甲酮皮肤释放量分别为 10.02%、9.04% 和 5.19%。不过，[14C]-二苯甲酮的质量平衡较低，分别为 81.5%、85.3% 和 8.02%。仅对防晒霜配方进行了挥发性测试，用铝箔代替皮肤。用药时，[14C]-二苯甲酮的质量平衡为 99%，但在用药后 24 小时内，[14C]-二苯甲酮的质量平衡分别降至 56.9% 和 60.6%，这证实了[14C]-二苯甲酮的挥发造成了损失。建议采用 12.42% 的保守皮肤吸收值来涵盖含有[14C]-二苯甲酮的制剂。

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来源期刊

Toxicology in Vitro 医学-毒理学

CiteScore

6.50

自引率

3.10%

发文量

181

审稿时长

65 days

期刊介绍： Toxicology in Vitro publishes original research papers and reviews on the application and use of in vitro systems for assessing or predicting the toxic effects of chemicals and elucidating their mechanisms of action. These in vitro techniques include utilizing cell or tissue cultures, isolated cells, tissue slices, subcellular fractions, transgenic cell cultures, and cells from transgenic organisms, as well as in silico modelling. The Journal will focus on investigations that involve the development and validation of new in vitro methods, e.g. for prediction of toxic effects based on traditional and in silico modelling; on the use of methods in high-throughput toxicology and pharmacology; elucidation of mechanisms of toxic action; the application of genomics, transcriptomics and proteomics in toxicology, as well as on comparative studies that characterise the relationship between in vitro and in vivo findings. The Journal strongly encourages the submission of manuscripts that focus on the development of in vitro methods, their practical applications and regulatory use (e.g. in the areas of food components cosmetics, pharmaceuticals, pesticides, and industrial chemicals). Toxicology in Vitro discourages papers that record reporting on toxicological effects from materials, such as plant extracts or herbal medicines, that have not been chemically characterized.