Spinosad-induced intergenerational sublethal effects on Tuta absoluta: biological traits and related genes expressions

IF 5.6 1区农林科学 Q1 ENTOMOLOGY

Entomologia Generalis Pub Date : 2024-05-02 DOI:10.1127/entomologia/2024/2452

Farman Ullah, Ali Güncan, Hina Gul, Muhammad Hafeez, Shuxing Zhou, Yaru Wang, Zhijun Zhang, Jun Huang, Hamed A. Ghramh, Wenchao Guo, Kaiyun Fu, Xiaowei Li, Nicolas Desneux, Yaobin Lu

{"title":"Spinosad-induced intergenerational sublethal effects on Tuta absoluta: biological traits and related genes expressions","authors":"Farman Ullah, Ali Güncan, Hina Gul, Muhammad Hafeez, Shuxing Zhou, Yaru Wang, Zhijun Zhang, Jun Huang, Hamed A. Ghramh, Wenchao Guo, Kaiyun Fu, Xiaowei Li, Nicolas Desneux, Yaobin Lu","doi":"10.1127/entomologia/2024/2452","DOIUrl":null,"url":null,"abstract":"The South American tomato pinworm, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), is considered one of the most economically important invasive pests that causes severe damage to tomato crops. The biopesticide spinosad is widely used for controlling T. absoluta. This study aimed to investigate the spinosad-induced intergenerational sublethal effects on T. absoluta. Results showed that spinosad exhibited high toxicity against 3rd instar T. absoluta with the LC50 of 0.04 mg L-1 after 72 h exposure. The LC10 and LC30 of spinosad significantly decreased the durations of various developmental stages and some reproductive parameters of parental (F0) T. absoluta compared to control. The developmental durations, survival rate, reproduction and life table parameters (intrinsic rate of increase, r; finite rate of increase, λ; net reproductive rate, R 0; and oviposition days, Od ) were significantly decreased in the progeny generations (F1 and F2) following F0 exposure to the LC10 and LC30 of spinosad as compared to the control group. Additionally, the mRNA expression levels of development and reproduction related genes such as Vg, VgR, and JHBP were down-regulated in parental and progeny generations at both spinosad treatments as compared to control treatment. The resistance related cytochrome P450 genes CYP321C40 and CYP6AW1, followed by CYP4M116, CYP9A307v2, CYP15C1, CYP339A1, and CYP6AB327 were up-regulated at spinosad-treated groups in the parental and progeny generations of T. absoluta as compared to control. Overall, these results showed that although spinosad exhibited high toxicity and induces intergenerational sublethal effects on the parental and progeny generations, the indiscriminate and long-term application might causes resistance development in of T. absoluta.","PeriodicalId":11728,"journal":{"name":"Entomologia Generalis","volume":"97 1","pages":""},"PeriodicalIF":5.6000,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Entomologia Generalis","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1127/entomologia/2024/2452","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENTOMOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

The South American tomato pinworm, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), is considered one of the most economically important invasive pests that causes severe damage to tomato crops. The biopesticide spinosad is widely used for controlling T. absoluta. This study aimed to investigate the spinosad-induced intergenerational sublethal effects on T. absoluta. Results showed that spinosad exhibited high toxicity against 3rd instar T. absoluta with the LC50 of 0.04 mg L-1 after 72 h exposure. The LC10 and LC30 of spinosad significantly decreased the durations of various developmental stages and some reproductive parameters of parental (F0) T. absoluta compared to control. The developmental durations, survival rate, reproduction and life table parameters (intrinsic rate of increase, r; finite rate of increase, λ; net reproductive rate, R 0; and oviposition days, Od ) were significantly decreased in the progeny generations (F1 and F2) following F0 exposure to the LC10 and LC30 of spinosad as compared to the control group. Additionally, the mRNA expression levels of development and reproduction related genes such as Vg, VgR, and JHBP were down-regulated in parental and progeny generations at both spinosad treatments as compared to control treatment. The resistance related cytochrome P450 genes CYP321C40 and CYP6AW1, followed by CYP4M116, CYP9A307v2, CYP15C1, CYP339A1, and CYP6AB327 were up-regulated at spinosad-treated groups in the parental and progeny generations of T. absoluta as compared to control. Overall, these results showed that although spinosad exhibited high toxicity and induces intergenerational sublethal effects on the parental and progeny generations, the indiscriminate and long-term application might causes resistance development in of T. absoluta.

查看原文本刊更多论文

Spinosad 对 Tuta absoluta 的代间亚致死效应：生物特征和相关基因的表达

南美番茄蛲虫 Tuta absoluta (Meyrick) （鳞翅目：Gelechiidae）被认为是对番茄作物造成严重危害的最重要的经济入侵害虫之一。生物农药 spinosad 被广泛用于防治 T. absoluta。本研究旨在探讨spinosad对T. absoluta的代际亚致死效应。结果表明，暴露 72 小时后，尖孢噻螨对第三龄绝对褐翅蝶的半数致死浓度（LC50）为 0.04 毫克/升，表现出较高的毒性。与对照组相比，LC10和LC30能显著降低亲本（F0）T. absoluta各发育阶段的持续时间和一些生殖参数。与对照组相比，F0暴露于刺五加LC10和LC30后，其后代（F1和F2）的发育持续时间、存活率、繁殖和生命表参数（固有增长率r、有限增长率λ、净繁殖率R 0和产卵天数Od）均显著降低。此外，与对照组相比，亲代和后代中与发育和繁殖相关的基因（如 Vg、VgR 和 JHBP）的 mRNA 表达水平在两种尖孢霉素处理下均出现下调。与对照组相比，抗性相关的细胞色素 P450 基因 CYP321C40 和 CYP6AW1，以及 CYP4M116、CYP9A307v2、CYP15C1、CYP339A1 和 CYP6AB327 在刺吸式杀虫剂处理组的亲代和后代中上调。总之，这些结果表明，虽然旋覆花对亲代和子代具有高毒性和诱导代间亚致死效应，但长期滥用旋覆花可能会导致 T. absoluta 产生抗性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Entomologia Generalis 生物-昆虫学

CiteScore

7.10

自引率

18.80%

发文量

审稿时长

>12 weeks

期刊介绍： Its scope covers all aspects of basic and applied research dealing with insects and more broadly with arthropods inhabiting wild, agricultural and/or urban habitats. The journal also considers research integrating various disciplines and issues within the broad field of entomology and ecology. Entomologia Generalis publishes high quality research articles on advances in knowledge on the ecology and biology of arthropods, as well as on their importance for key ecosystems services, e.g. as biological control and pollination. The journal devotes special attention to contributions providing significant advances (i) on the fundamental knowledge and on sustainable control strategies of arthropod pests (including of stored products) and vectors of diseases, (ii) on the biology and ecology of beneficial arthropods, (iii) on the spread and impact of invasive pests, and (iv) on potential side effects of pest management methods. Entomologia Generalis welcomes review articles on significant developments in the field of entomology. These are usually invited by the editorial board, but proposals may be sent to the Editor-in-Chief for preliminary assessment by the editorial board before formal submission to the journal. The journal also considers comments on papers published in Entomologia Generalis, as well as short notes on topics that are of broader interest.