Dietary chromium-methionine supplementation and broiler (22–43 days) responses during heat stress. 2 - Physiological variables, and heat shock protein 70 and insulin-like growth factor-1 gene expression

IF 1.4 4区农林科学 Q2 Agricultural and Biological Sciences

Animal Production Science Pub Date : 2024-05-01 DOI:10.1071/an23354

Felipe Santos Dalólio, Luiz Fernando Teixeira Albino, Haniel Cedraz de Oliveira, Alba Kyonara Barbosa Alves Tenorio Fireman, Alvaro Burin Junior, Marcos Busanello, Nilton Rohloff Junior, Guilherme Luis Silva Tesser, Ricardo Vianna Nunes

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引用次数: 0

Abstract

Context

Dietary supplementation with trace mineral chromium (Cr) has been shown to enhance the physiological responses of broilers subjected to heat stress (HS), modulate gene expression, and improve performance.

Aims

This study aimed to evaluate the impact of chromium–methionine (CrMet) supplementation on growth performance, body temperatures, lymphoid organ weights, hormones, blood parameters, and the expression of heat-shock protein-70 (HSP-70) and insulin-like growth factor-1 (IGF-1) genes in broilers under HS conditions (33°C for 12 h/day).

Methods

In the first experiment, 336 22-day-old male broilers were randomly distributed into four blocks with six treatments (0, 0.10, 0.20, 0.40, 0.80, and 1.20 mg/kg CrMet) and eight replicates with seven birds per cage. These broilers were subjected to HS from 22 to 43 days of age. In the second experiment, 24 male broilers, in total, at 43 days of age, previously exposed to HS, were randomly distributed to the same six treatments from the first experiment, with four replicates. Breast samples were collected for the analysis of HSP-70 and IGF-1 expression.

Results

A quadratic effect (P < 0.05) was observed on bodyweight gain (BWG) and feed conversion ratio (FCR). The supplementation of 0.71 and 0.68 mg/kg improved BWG and FCR, respectively. At 28 days of age, cloacal and mean body temperatures, corticosterone, and thyroid hormones were quadratically affected (P < 0.05), while at 43 days of age, a linear effect (P < 0.05) was observed on haemoglobin concentration. There was a reduction (P < 0.05) in the expression of HSP-70 and an increase in IGF-1 (P < 0.05) in the breast tissue of broilers supplemented with CrMet.

Conclusions

The supplementation with 0.71 mg/kg and 0.68 mg/kg of CrMet improved BWG and FCR, respectively. Additionally, the supplementation with 0.80 mg/kg improved hormones, reduced HSP-70 and increased the expression of IGF-1 in broilers during HS.

Implications

These findings suggest that CrMet can be included in the diet of broiler chickens subjected to HS to enhance physiological responses and performance.

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膳食铬蛋氨酸补充剂与肉鸡（22-43 天）在热应激期间的反应。2 - 生理变量以及热休克蛋白 70 和胰岛素样生长因子-1 基因的表达

背景研究表明，膳食中补充微量矿物质铬（Cr）可增强肉鸡在热应激（HS）下的生理反应、调节基因表达并提高生长性能。目的本研究旨在评估在热应激条件下（33°C，12 小时/天）补充铬蛋氨酸（CrMet）对肉鸡生长性能、体温、淋巴器官重量、激素、血液参数以及热休克蛋白-70（HSP-70）和胰岛素样生长因子-1（IGF-1）基因表达的影响。方法在第一项实验中，将 336 只 22 日龄的雄性肉鸡随机分为四个区组，六个处理（0、0.10、0.20、0.40、0.80 和 1.20 mg/kg CrMet），八个重复，每个笼子七只。这些肉鸡在 22 至 43 日龄期间接受 HS 试验。在第二项实验中，总共 24 只 43 日龄的雄性肉鸡被随机分配到第一项实验中的 6 种处理中，每笼 4 个重复。收集乳房样本用于分析 HSP-70 和 IGF-1 的表达。结果在体重增加（BWG）和饲料转化率（FCR）上观察到了二次效应（P < 0.05）。0.71和0.68 mg/kg的添加量分别提高了体重增重和饲料转化率。28 日龄时，泄殖腔温度和平均体温、皮质酮和甲状腺激素受到二次影响（P < 0.05）；43 日龄时，血红蛋白浓度受到线性影响（P < 0.05）。在添加 CrMet 的肉鸡乳腺组织中，HSP-70 的表达量减少（P <0.05），IGF-1 的表达量增加（P <0.05）。结论添加 0.71 毫克/千克和 0.68 毫克/千克的 CrMet 可分别提高肉鸡的体重和饲料报酬率。此外，添加 0.80 毫克/千克的 CrMet 可改善激素水平，降低 HSP-70 并增加 HS 期间肉鸡 IGF-1 的表达。启示这些研究结果表明，可将 CrMet 添加到遭受 HS 的肉鸡日粮中，以提高其生理反应和生产性能。

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来源期刊

Animal Production Science Agricultural and Biological Sciences-Food Science

CiteScore

3.00

自引率

7.10%

发文量

139

审稿时长

3-8 weeks

期刊介绍： Research papers in Animal Production Science focus on improving livestock and food production, and on the social and economic issues that influence primary producers. The journal (formerly known as Australian Journal of Experimental Agriculture) is predominantly concerned with domesticated animals (beef cattle, dairy cows, sheep, pigs, goats and poultry); however, contributions on horses and wild animals may be published where relevant. Animal Production Science is published with the endorsement of the Commonwealth Scientific and Industrial Research Organisation (CSIRO) and the Australian Academy of Science.