Evaluation of proteome dynamics: Implications for statistical confidence in mass spectrometric determination

Abstract

Single-cell proteomics is currently far less productive than other approaches. Still, the proteomic community is having trouble adapting to the limitation of having to examine fewer cells than they would like. Studies on a small number of cells should be carefully planned to maximize the chances of success in this situation. This study aims to determine how sample size and measurement speed (slope)/variation affect the accuracy of a protein proteome mass spectrometric determination. The determination accuracy was shown to increase, and the false positive rate was shown to decrease as the sample size increased from 7 to 100 cells and the measurement slope/variation (S/V) ratio increased from 1 to 6. Furthermore, it was discovered that the number of cells in the sample increased the accuracy of this estimate. Thus, for 100 cells, the measurement S/V ratio was typically estimated to be very close to the real-world value, with a standard deviation of 0.35. For sample sizes from 7 to 100 cells, this accuracy was seen when calculating the measurement S/V ratio. The findings can help researchers plan experiments for mass spectroscopic protein proteome determination and other research purposes.