Salivary levels of Cathelicidin LL-37 in patients with oral potentially malignant lesions, A case control study

N. Tarrad, Sandy Shaaban, Olfat Shaker, Mai Zakaria
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Abstract

Aim : The recognition of practical early diagnostic biomarkers is a cornerstone of improved prevention and treatment of cancer thus the current study estimated salivary level of Cathelicidin LL-37 in patients suffering from potentially malignant lesions and control subjects to corroborate Cathelicidin LL-37 as a diagnostic marker for early detection of potentially malignant diseases and revealing its possible role in carcinogenesis. Methodology : 45 systemically healthy individuals were subdivided into three groups: Group I: 15 Healthy participants without any oral lesions. Group II: 15 Patients having atrophic/erosive oral lichen planus (OLP). Group III: 15 Patients having oral leukoplakia. Enzyme linked immune-sorbent assay (ELIZA) kit was used to evaluate the level of LL-37 in whole unstimulated salivary samples collected from all participants. To reveal AUC, sensitivity, specificity, and diagnostic accuracy of LL-37 receiver operating curve (ROC) analysis was done. Results : The highest salivary level of LL-37 was revealed in OLP patients followed by oral leukoplakia patients whereas it was the lowest in healthy controls. ROC analysis exhibited excellent diagnostic accuracy of salivary LL-37 in differentiating both OLP and leukoplakia from control and OLP from leukoplakia. Conclusions : LL-37 appears to have a potential role in potentially malignant lesions (OLP & leukoplakia). The remarkable diagnostic accuracy of salivary LL-37 in differentiating potentially malignant lesion and healthy control could confirm its utilization as an innovative marker to early diagnose potentially malignant lesions. Salivary LL-37 being non-invasive accurate marker could be a chair-side diagnostic method that detect potentially malignant lesions. Clinical relevance: Salivary Cathelicidin LL-37 being non-invasive could serve as a chair-side diagnostic technique for potentially malignant lesions.
口腔潜在恶性病变患者唾液中的卡他列汀 LL-37 水平,一项病例对照研究
目的:识别实用的早期诊断生物标志物是改善癌症预防和治疗的基石,因此,本研究估测了潜在恶性病变患者和对照组唾液中卡特里西汀 LL-37 的水平,以证实卡特里西汀 LL-37 是早期检测潜在恶性疾病的诊断标志物,并揭示其在致癌过程中可能发挥的作用。方法:将 45 名全身健康者分为三组:第一组:15 名无任何口腔病变的健康参与者。第二组:15 名患有萎缩性/侵蚀性口腔扁平苔藓(OLP)的患者。第三组:15 名口腔白斑病患者。使用酶联免疫吸附试验(ELIZA)试剂盒评估从所有参与者采集的未刺激唾液样本中的 LL-37 含量。为了揭示 LL-37 的 AUC、灵敏度、特异性和诊断准确性,进行了接收操作曲线(ROC)分析。结果:OLP 患者的唾液中 LL-37 含量最高,其次是口腔白斑病患者,而健康对照组的唾液中 LL-37 含量最低。ROC分析表明,唾液LL-37在区分OLP和白斑病与对照组以及OLP与白斑病方面具有极高的诊断准确性。结论 :LL-37 似乎在潜在恶性病变(OLP 和白斑病)中具有潜在作用。唾液 LL-37 在区分潜在恶性病变和健康对照方面的诊断准确性很高,这证实了唾液 LL-37 是早期诊断潜在恶性病变的创新标记物。唾液 LL-37 是一种非侵入性的准确标记物,可作为一种检测潜在恶性病变的椅旁诊断方法。临床意义:唾液中的白细胞介素 LL-37 是非侵入性的,可作为潜在恶性病变的辅助诊断技术。
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