Unveiling thymosin antimicrobial peptide from pearl spot, Etroplus suratensis: Molecular characterization, phylogenetic analysis, and functional implications

Q3 Agricultural and Biological Sciences

Ecological Genetics and Genomics Pub Date : 2024-04-24 DOI:10.1016/j.egg.2024.100253

Kavya Gokul, Raniya Samad, Anne Maria Thomas, Swapna P. Antony

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引用次数: 0

Abstract

Background

Antimicrobial peptides are small, cationic, amphipathic peptides composed of 10–50 amino acids, playing a crucial role in innate immunity. While they are found in both vertebrates and invertebrates, they are particularly effective in invertebrates. This study presents the identification and molecular characterization of the potential antimicrobial peptide thymosin from Pearl spot, Etroplus suratensis (Es-Thyβ). Phylogenetic analysis and structural characterization of the identified peptide are analyzed and substantiated through the study.

Results

The sequence analysis of Es-Thyβ revealed an Open Reading Frame (ORF) of 132 nucleotides, encoding a 43-amino acid peptide. The hydrophobicity of the peptide, determined using PepDraw, was found to be +68.93 Kcal*mol⁻¹. Through in silico analysis the identified Thymosin was determined to be Es-Thyβ. The structure and characteristics of the peptide sequence were further analyzed using multiple in silico software tools, providing confirmation of its identity as Es-Thyβ.

Conclusion

The study identified and described a potential antimicrobial peptide Es-Thyβ, a member of the thymosin β4 family, with conserved sequences and evolutionary links across teleost fishes.

查看原文本刊更多论文

揭开珍珠斑（Etroplus suratensis）胸腺素抗菌肽的神秘面纱：分子特征、系统发育分析和功能影响

背景抗菌肽是由 10-50 个氨基酸组成的小型阳离子两性肽，在先天性免疫中发挥着至关重要的作用。虽然脊椎动物和无脊椎动物中都有抗菌肽，但它们在无脊椎动物中尤其有效。本研究对珍珠斑（Etroplus suratensis）中潜在的抗菌肽胸腺肽（Es-Thyβ）进行了鉴定和分子表征。结果Es-Thyβ的序列分析显示，其开放阅读框（ORF）包含132个核苷酸，编码43个氨基酸肽。使用 PepDraw 测定的肽疏水性为 +68.93 Kcal*mol-1。通过硅分析，确定胸腺肽为 Es-Thyβ。该研究发现并描述了一种潜在的抗菌肽 Es-Thyβ，它是胸腺肽 β4家族的成员，其序列和进化关系在长尾鱼类中是保守的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Ecological Genetics and Genomics Agricultural and Biological Sciences-Ecology, Evolution, Behavior and Systematics

CiteScore

1.80

自引率

0.00%

发文量

期刊介绍： Ecological Genetics and Genomics publishes ecological studies of broad interest that provide significant insight into ecological interactions or/ and species diversification. New data in these areas are published as research papers, or methods and resource reports that provide novel information on technologies or tools that will be of interest to a broad readership. Complete data sets are shared where appropriate. The journal also provides Reviews, and Perspectives articles, which present commentary on the latest advances published both here and elsewhere, placing such progress in its broader biological context. Topics include: -metagenomics -population genetics/genomics -evolutionary ecology -conservation and molecular adaptation -speciation genetics -environmental and marine genomics -ecological simulation -genomic divergence of organisms