Alpha-amylase Inhibitory and Cytotoxicity Investigation of Biologically Synthesized Silver Nanoparticles Using Balanites aegyptiaca on C2C12 Cells

Abstract

Silver nanoparticles were biologically synthesized from the leaf and seed extract of Balanites aegyptiaca against diabetes. The silver nanoparticles were characterized using UV-visible spectroscopy, FTIR, SEM, TEM, zeta potential and dynamic light scattering for size distribution. The finding proved the nanosize and spherical shapes of the nanoparticles. In-vitro antidiabetic and cytotoxic activities of the synthesized nanoparticles were evaluated for the first time in both the plant extract, and they showed significant inhibition of α-amylase. However, the inhibition was elevated in the case of nanoparticles. It was found that seed and leaf extract inhibited α-amylase up to 62.23±7.25631% and 63.90±5.632%, respectively. The elevation in the inhibition was observed in the case of their respective silver nanoparticles, such as AgNP seeds and AgNP leaves inhibited the enzyme up to 72.71±7.8569% and 74.04±6.3254%, respectively. They showed lesser cytotoxicity against C2C12 cells. It can be concluded that the leaf and seed extracts of Balanites aegyptiaca and synthesized silver nanoparticles from this plant can be good anti-diabetic agents.