Anti-inflammatory effects of eggshell membrane hydrolysates on carrageenan-induced rat

IF 0.5 Q4 EDUCATION, SCIENTIFIC DISCIPLINES
S. Vikasari, E. Sukandar, Tri Suciati, I. Adnyana
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引用次数: 0

Abstract

Background: Eggshells are one of the most common culinary wastes, yet using eggshell membrane detritus in medical treatments remains largely unexplored. Objective: This study aims to assess the anti-inflammatory potential of eggshell membrane hydrolysate. Method: Eggshell membrane hydrolysate was prepared using acid-base hydrolysis. The intraplantar carrageenan-induced inflammation model was used to test the anti-inflammatory effect. The experimental group consisted of a control, comparison (4.5 mg/kg BW diclofenac-Na, 4.5 mg/kg BW hyaluronic acid, and 45 mg/kg BW collagen), and eggshell membrane hydrolysates (9, 22.5, 45 and 90 mg/kg BW). Observations of inflammation, percentage of inflammation, and percentage of inhibition of inflammation were measured every 30 minutes for 180 minutes. Statistical analysis was performed using the t-test and also trendline analysis. Result: The findings revealed that collagen and hyaluronic acid demonstrated the ability to suppress inflammation compared to controls (p < 0.05). Compared to the control group, the group that received eggshell membrane hydrolysate at doses of 9, 22.5, 45, and 90 mg/kg BW also exhibited inhibition of inflammation (p < 0.05). Furthermore, increasing the dosage of eggshell membrane hydrolysate correlated with an increase in the percentage of inflammation inhibition in carrageenan-induced rats. Conclusion: Eggshell membrane hydrolysate has the potential to act as an anti-inflammatory agent.
蛋壳膜水解物对卡拉胶诱导的大鼠的抗炎作用
背景:蛋壳是最常见的烹饪废弃物之一,但将蛋壳膜碎片用于医学治疗在很大程度上仍未得到探索:本研究旨在评估蛋壳膜水解物的抗炎潜力:方法:采用酸碱水解法制备蛋壳膜水解物。方法:采用酸碱水解法制备蛋壳膜水解物,并采用角叉菜胶诱导的跖内炎模型测试其抗炎效果。实验组包括对照组、对比组(4.5 毫克/千克体重双氯芬那、4.5 毫克/千克体重透明质酸和 45 毫克/千克体重胶原蛋白)和蛋壳膜水解物组(9、22.5、45 和 90 毫克/千克体重)。在 180 分钟内,每隔 30 分钟测量一次炎症观察值、炎症百分比和炎症抑制百分比。统计分析采用 t 检验和趋势线分析:结果:研究结果表明,与对照组相比,胶原蛋白和透明质酸具有抑制炎症的能力(P < 0.05)。与对照组相比,剂量为 9、22.5、45 和 90 mg/kg BW 的蛋壳膜水解物组也表现出了抑制炎症的作用(p < 0.05)。此外,增加蛋壳膜水解物的剂量与角叉菜胶诱导的大鼠炎症抑制百分比的增加相关:结论:蛋壳膜水解物具有抗炎潜力。
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来源期刊
Pharmacy Education
Pharmacy Education EDUCATION, SCIENTIFIC DISCIPLINES-
CiteScore
0.80
自引率
20.00%
发文量
174
期刊介绍: Pharmacy Education journal provides a research, development and evaluation forum for communication between academic teachers, researchers and practitioners in professional and pharmacy education, with an emphasis on new and established teaching and learning methods, new curriculum and syllabus directions, educational outcomes, guidance on structuring courses and assessing achievement, and workforce development. It is a peer-reviewed online open access platform for the dissemination of new ideas in professional pharmacy education and workforce development. Pharmacy Education supports Open Access (OA): free, unrestricted online access to research outputs. Readers are able to access the Journal and individual published articles for free - there are no subscription fees or ''pay per view'' charges. Authors wishing to publish their work in Pharmacy Education do so without incurring any financial costs.
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