Multiple trait comparison and global intestine transcriptional provide new insights into bases of heterosis in hybrid tilapia (Oreochromis niloticus × Oreochromis aureus)

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Zihui Liu , Binglin Chen , Zhiying Zou , Dayu Li , Jinglin Zhu , Jie Yu , Wei Xiao , Hong Yang
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引用次数: 0

Abstract

Heterosis has been utilized in aquaculture for many years, yet its molecular basis remains elusive. Therefore, a comprehensive analysis of heterosis was conducted by comparing growth, digestion and biochemistry indices, as well as the intestinal gene expression profiles of Nile tilapia, blue tilapia and their hybrids. The results revealed that hybrid tilapia demonstrated an enhanced growth traits and elevated digestive enzyme activity compared to Nile and blue tilapia. Additionally, the hybrid tilapia displayed superior antioxidants and non-specific immune levels, with increased levels of catalase (CAT), alkaline phosphatase (AKP), acid phosphatase (ACP), glutathione (GSH), superoxide dismutase (SOD), total antioxidant capacity (TAOC), lysozyme, and immunoglobulin M (IgM) relative to Nile and blue tilapia. Moreover, 3392, 2470 and 1261 differentially expressed genes (DEGs) were identified in the intestinal tissues when comparing Nile tilapia to blue tilapia, hybrid tilapia to blue tilapia, and hybrid tilapia to Nile tilapia. Upon classifying the differentially expressed genes (DEGs), non-additively expressed DEGs accounted for 68.1 % of the total DEGs, with dominant and over-dominant expressed DEGs comprising 63.7 % and 4.4 % in the intestines, respectively. These non-additively expressed DEGs were primarily associated with metabolic, digestive, growth, and developmental pathways. This enrichment enhances our comprehension of the molecular underpinnings of growth heterosis in aquatic species.

Abstract Image

多性状比较和全球肠道转录为杂交罗非鱼(Oreochromis niloticus × Oreochromis aureus)的杂交基础提供了新见解
杂交在水产养殖中已应用多年,但其分子基础仍然难以捉摸。因此,我们通过比较尼罗罗非鱼、蓝罗非鱼及其杂交种的生长、消化和生化指标以及肠道基因表达谱,对杂交现象进行了全面分析。结果显示,与尼罗罗非鱼和蓝罗非鱼相比,杂交罗非鱼的生长性状增强,消化酶活性提高。此外,与尼罗罗非鱼和蓝罗非鱼相比,杂交罗非鱼的过氧化氢酶(CAT)、碱性磷酸酶(AKP)、酸性磷酸酶(ACP)、谷胱甘肽(GSH)、超氧化物歧化酶(SOD)、总抗氧化能力(TAOC)、溶菌酶和免疫球蛋白M(IgM)的水平均有所提高,显示出优异的抗氧化能力和非特异性免疫水平。此外,在尼罗罗非鱼与蓝罗非鱼、杂交罗非鱼与蓝罗非鱼、杂交罗非鱼与尼罗罗非鱼的肠道组织中,分别发现了3392、2470和1261个差异表达基因(DEGs)。在对差异表达基因(DEGs)进行分类后,非重复表达的 DEGs 占 DEGs 总数的 68.1%,肠道中显性和过显性表达的 DEGs 分别占 63.7% 和 4.4%。这些非重复表达的 DEGs 主要与代谢、消化、生长和发育途径有关。这种丰富性增强了我们对水生物种生长异质性分子基础的理解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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