Antibacterial Effect of Nanoparticles Synthesized from Ficus exaseprata (Sandpaper Leaves)

Asian Journal of Applied Chemistry Research Pub Date : 2024-04-02 DOI:10.9734/ajacr/2024/v15i1280

H. A. Orlu, Amba Ollor Ollor, Eze Saint Ebor

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Abstract

The eco-friendly, cost effectiveness, and the less toxic nature of synthesized green nanoparticles to the environment have become a global attraction to many studies. The study aim to synthesize Silver (Ag) nanoparticles from Ficus exasperata (Sandpaper leave) leaves and to determine its antimicrobial effect on bacteria isolates. Silver (Ag) nanoparticles were synthesized from the plant extracts using standard extracting techniques and their presence was verified and confirmed using an ultraviolet-visible (UV-Vis) Spectrophotometer. A prepared series of silver nitrate (AgNO3) solutions were mixed with the plant extracts at a ratio of 1:1 (v/v) to a total volume of 20ml in a text-tube. The test tubes were rapped with aluminum foil and heated in a water bath at 60OC for 3 hours and allowed to cool and analyzed using UV-Vis Spectrophotometer. The UV-Vis spectra confirmed the different concentrations of silver nitrate (AgNO3) that produced Ag nanoparticles and their average size was more than 50nm and less than 100nm. The mixture of the leave extract was tested for its antimicrobial activity against gram-positive and gram-negative organisms such as Staphylococcus aureus Bacillus cereus and Escherichia coli respectively. The results showed that the growth of the different bacteria was inhibited by the extracts containing silver nitrate on Staphylococcus aureus and Escherichia coli and not on Bacillus cereus using Ficus exaseprata nanoparticles. Statistical evaluation showed that zones of inhibition on the three bacteria produced by the aqueous leave extracts containing different concentration of silver nitrate (AgNO3¬¬) precursor was significantly different from the silver nitrate (AgNO3) precursor. It were observed that the higher the concentration of the silver nitrate (AgNO3) the greater the zone of inhibition. It can be concluded that Ficus exaseprata leave extracts dope with silver nanoparticles, can produce antimicrobial effects on some microorganisms.

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用砂纸叶合成的纳米粒子的抗菌效果

合成的绿色纳米粒子具有生态友好、成本效益高、对环境毒性小等特点，在全球范围内吸引了众多研究人员的关注。本研究旨在从榕树叶中合成银（Ag）纳米粒子，并确定其对细菌分离物的抗菌效果。使用标准提取技术从植物提取物中合成了银（Ag）纳米粒子，并使用紫外可见分光光度计验证和确认了银纳米粒子的存在。将制备好的一系列硝酸银（AgNO3）溶液与植物提取物按 1:1 (v/v) 的比例混合，装入总体积为 20 毫升的试管中。试管用铝箔包好，在 60OC 水浴中加热 3 小时，冷却后使用紫外可见分光光度计进行分析。紫外可见光谱证实了不同浓度的硝酸银（AgNO3）产生了银纳米粒子，其平均粒径大于 50 纳米，小于 100 纳米。对叶提取物的混合物进行了抗菌活性测试，分别针对金黄色葡萄球菌、蜡样芽孢杆菌和大肠杆菌等革兰氏阳性和阴性菌。结果表明，含有硝酸银的萃取物对金黄色葡萄球菌和大肠杆菌有抑制作用，但对蜡样芽孢杆菌没有抑制作用。统计评估表明，含有不同浓度硝酸银（AgNO3¬¬）前体的水提取物对三种细菌产生的抑菌区与硝酸银（AgNO3）前体有显著差异。据观察，硝酸银（AgNO3）浓度越高，抑制区越大。由此可以得出结论，银杏叶提取物掺入纳米银粒子后，可以对某些微生物产生抗菌作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Asian Journal of Applied Chemistry Research

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