Application of Membrane-less Microbial Fuel Cell in Reducing Human Hazards from Dewatered Sludge

Pub Date : 2024-04-04 DOI:10.47836/pjst.32.3.12

Fatin Nur Izzati Mohd Fadzil, Chen Sep Ngee, Mohammed Zharif Asyrani Mohammed Alias, Muhammad Adib Fadhlullah Muhammad Lukman, A. S. Yaakop, Muaz Mohd ZAINI MAKHTAR, Ana Masara Ahmad Mokhtar

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Abstract

Membrane-less microbial fuel cell (ML-MFC) technology has emerged as a potential for wastewater treatment and electricity generation. Despite its benefit in green energy production, studies have yet to determine its role in minimizing the human hazards stemming from dewatered sludge (DS). Hence, this research aims to investigate the effects of ML-MFC-treated DS on cell toxicity and its benefits in reducing protein-denaturation-related inflammation and antimicrobial resistance (AMR) dissemination. MTT assay was performed to determine the cytotoxic effect of ML-MFC-treated DS on 3T3-L1 and Hep G2 cells at 24 h. The anti-inflammatory property of ML-MFC-treated DS was determined using a protein denaturation assay. Next, the antibiotic susceptibility of bacteria isolated from ML-MFC-treated samples was determined using the disk-diffusion method. All the data obtained were statistically analyzed using GraphPad Prism software (Version 9.2.0) with a p-value ≤0.05 was considered significant. Interestingly, ML-MFC-treated DS showed 80% cell viability on 3T3-L1 and slight toxicity on Hep G2 cells. ML-MFC-treated DS exhibited anti-inflammatory properties with 62.43% protein denaturation inhibition and displayed fewer antibiotic-resistance bacteria than the untreated. Overall, the ML-MFC technology showed novel applications by decreasing DS-related health hazards.

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无膜微生物燃料电池在减少脱水污泥对人体危害中的应用

无膜微生物燃料电池（ML-MFC）技术已成为污水处理和发电的潜在技术。尽管该技术在绿色能源生产方面大有裨益，但在最大限度地减少脱水污泥（DS）对人类的危害方面，相关研究尚未确定其作用。因此，本研究旨在调查 ML-MFC 处理过的脱水污泥对细胞毒性的影响，以及它在减少蛋白质变性相关炎症和抗菌剂耐药性（AMR）传播方面的益处。MTT 试验测定了 ML-MFC 处理过的 DS 在 24 小时内对 3T3-L1 和 Hep G2 细胞的细胞毒性作用。然后，使用盘扩散法测定了从 ML-MFC 处理过的样品中分离出来的细菌对抗生素的敏感性。所有数据均使用 GraphPad Prism 软件（9.2.0 版）进行统计分析，P 值≤0.05 为显著。有趣的是，ML-MFC 处理的 DS 对 3T3-L1 细胞的存活率为 80%，而对 Hep G2 细胞则有轻微毒性。经 ML-MFC 处理的 DS 具有抗炎特性，蛋白质变性抑制率为 62.43%，与未经处理的 DS 相比，抗生素耐药菌更少。总之，ML-MFC 技术减少了与 DS 相关的健康危害，显示了其新颖的应用前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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