Assessment of the toxic effect of the medical glue «Sulfacrylate» in M-22 cell culture

Abstract

Sulfacrylate is a medical glue used in general and vascular surgery, traumatology, maxillofacial surgery, thoracic and abdominal surgery, and dentistry. To connect tissues in plastic ophthalmic surgery, the toxic properties of Sulfacrylate should be studied. AIM: To evaluate the toxic effect of Sulfacrylate in human cell culture in vitro. MATERIAL AND METHODS: In vitro studies were conducted on human fibroblast cultures of the M-22 line from the 20th passage. Sulfacrylate was used in the present study (registration certificate for a medical device no. FSR 2010/09805; December 31, 2010). A lyophilized bandage based on human type 1 collagen was used to fix the glue. The total number of cells at the bottom of wells, cell density at the bottom of wells and on collagen dressings (1 thousand cells per cm2), structural integrity of cells and their morphology, integrity of cell membranes were evaluated. RESULTS: The toxic effect of collagen dressings with Sulfacrylate was limited by the size of the bandage. Collagen dressings with Sulfacrylate did not affect the viability of cells not in direct contact with the dressings and did not cause a pronounced decrease in the proliferative activity of cells. The minimum optimal dosage of adhesive for plastic ophthalmic surgery was 1–3 µkl. CONCLUSION: The use of Sulfacrylate in plastic ophthalmic surgery is feasible; however, the volume used should be minimized.