THE EFFECT OF AUTOMESOCONCENTRATE OF PLATELETS ON THE STAGES OF REGENERATION OF THE LIVER REMNANT OF RATS UNDER THE CONDITIONS OF PERFORMING VOLUMETRIC EXPERIMENTAL LIVER RESECTIONS

R. Salyutin, K.O. Yuzvyk
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Abstract

Introduction. Liver regeneration occurs in three stages, each of which is regulated by different factors: the initiation phase is regulated by interleukin 6 (IL-6) and tumor necrosis factor (TNFα); expansion phase – by hepatocyte growth factor (HGF) and transforming growth factor alpha (TGFα); the terminal phase – transforming growth factor beta (TGFβ) and activins. The work determined the level of markers of the main stages of liver regeneration after resection of 65-70% of the parenchyma of this organ in rats under the conditions of using different doses and schemes of administration of automesoconcentrate of platelets (AMС). The purpose of the study is to establish the characteristics of changes in the level of cytokines and growth factors in the homogenate of the liver of rats after resection of 65-70% of the parenchyma of this organ under the conditions of the use of different doses and schemes of AMС administration. Materials and methods. The study was conducted on white Wistar rats aged 1.5-2 months, weighing 150-180 g. AMС was injected: into the parenchyma of the liver remnant during resection at a dose of 0.5 ml/kg (group I); into the tail vein in the postoperative period at a dose of 0.5 ml/kg 3 hours and 48 hours after liver resection (group II); into the tail vein in the postoperative period at a dose of 1.0 ml/kg 3 hours and 48 hours after resection. Research results and discussion. It was shown that on the 7th day after liver resection, the level of TNF-α and IL-6 increased in all experimental groups, followed by a decrease on the 10th day to control values in the group of animals that received AMA intravenously at a dose of 1 ml/kg masses of animals. It was established that the values of HGF and TGFα in the liver homogenate of animals that underwent liver resection were significantly higher than in the control group on the 7th day of the experiment. At the later stages of the experiment, a decrease in the levels of HGF and TGFα in the liver homogenate was observed in the animals of groups I and III, but the studied indicators did not reach control values. In animals of group II, indicators of HGF and TGFα content remained at the highest level. The increase in the content of TGFβ and activins in the homogenate of the liver of rats in all experimental groups indicates the intensification of the terminal phase of liver regeneration on the 10th day after liver resection. Conclusions. Therefore, under the action of a dose of АМС equal to 1 ml/kg, the regeneration processes occur most intensively, the peak of which occurs 7-10 days after the resection. Administration of AMA into the liver parenchyma at a lower dose of 0.5 ml/kg or intravenous administration of the same dose does not contribute to the complete restoration of regenerative processes in the liver, as indicated by increased levels of TGFα and HGF on the 10th day after liver resection and administration of AMA.
在实验性肝脏体积切除的条件下,血小板自动浓缩物对大鼠残肝再生阶段的影响
引言肝脏再生分为三个阶段,每个阶段由不同的因子调节:起始阶段由白细胞介素 6(IL-6)和肿瘤坏死因子(TNFα)调节;扩展阶段--由肝细胞生长因子(HGF)和转化生长因子α(TGFα)调节;终结阶段--由转化生长因子β(TGFβ)和活化素调节。这项研究确定了在使用不同剂量和方案的血小板自体浓缩物(AMС)的条件下,切除大鼠肝脏65-70%实质后肝脏再生主要阶段的标志物水平。本研究旨在确定在使用不同剂量和方案的 AMС 给药条件下,切除大鼠肝脏 65-70% 的实质组织后,肝脏匀浆中细胞因子和生长因子水平的变化特征。材料和方法研究对象是年龄为1.5-2个月,体重为150-180克的白色Wistar大鼠。AMС的注射方法为:在切除过程中以0.5毫升/千克的剂量注射到残余肝脏的实质中(I组);在术后3小时和48小时后以0.5毫升/千克的剂量注射到尾静脉中(II组);在术后3小时和48小时后以1.0毫升/千克的剂量注射到尾静脉中。研究结果与讨论。结果表明,在肝脏切除术后第 7 天,所有实验组的 TNF-α 和 IL-6 水平都有所上升,随后在第 10 天,静脉注射 AMA 剂量为 1 毫升/千克的动物组的 TNF-α 和 IL-6 水平降至对照组的水平。实验证实,在实验的第 7 天,接受肝脏切除术的动物肝脏匀浆中的 HGF 和 TGFα 值明显高于对照组。在实验后期,观察到 I 组和 III 组动物肝脏匀浆中的 HGF 和 TGFα 水平有所下降,但所研究的指标并未达到控制值。在 II 组动物中,HGF 和 TGFα 含量指标保持在最高水平。所有实验组大鼠肝脏匀浆中 TGFβ 和活化素含量的增加表明,在肝脏切除后第 10 天,肝脏再生的末期阶段加剧。结论因此,在相当于 1 毫升/千克的АМС剂量作用下,再生过程最为密集,其高峰出现在肝切除术后 7-10 天。以 0.5 毫升/千克的较低剂量向肝实质内注射 AMA 或静脉注射相同剂量的 AMA 并不能促进肝脏再生过程的完全恢复,肝脏切除和注射 AMA 后第 10 天的 TGFα 和 HGF 水平升高就表明了这一点。
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