Variability of the component composition of the essential oil of Artemisia salsoloides Willd. from natural populations of the flora of Dagestan

F. Vagabova, A. M. Aliev, G. Radzhabov, M. M. Mallaliev
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Abstract

To study the variability of the gathering locality and componen t composition of essential oil from the aerial part of a little‐studied species – Artemisia salsoloides Willd. depending on abiotic environmental factors, such as height above the level of the place of growth. Aerial parts of A. salsoloides were collected during the flowering phase in four Dagestan natural populations to obtain essential oil using the Clevenger method. To determine the component composition of the samples obtained of essential oils of A. salsoloides, chromatography‐mass spectrometric analysis was used. The components were identified using the NIST08 and FFNSC mass spectral libraries. In total, 55 compounds were identified in the essential oil samples, where monoterpenoids account for 59.92 % – 75.98 % and sesquiterpenoids – for 23.24 % to 37.81 %. At the same time, 15 major compounds were identified. Thus, it should be noted that there was found to be a wide range of values for monoterpenoids: β‐pinene, trans‐β‐ocimene, cis‐alloocimene, benzene, 2,4‐pentadinyl, and among sesquiterpenoids – α‐copaene, β‐sesquiphilandrene, spathulenol. The quantitative yield of essential oil in the studied samples varied from 0.68 % to 1.36 % of airdried raw materials, depending on the growth location of the species. Four separate populations of A. salsoloides were identified by means of cluster analysis based on the component composition of the studied essential oils. For the first time, the composition of the essential oil of the aerial part of wild Dagestan samples of A. salsoloides was studied as depending on an abiotic factor (height above sea level) of the environment. The results obtained of the component composition of the essential oil of A. salsoloides samples showed the presence of a number of valuable compounds, which allow the use of essential oils as their sources.
来自达吉斯坦天然植物群的沙蒿精油成分的变异性
研究一种鲜有研究的物种--沙蒿(Artemisia salsoloides Willd.)气生部分精油的采集地点和成分组成的变化,这取决于非生物环境因素,如高于生长地水平的高度。在达吉斯坦四个自然种群的开花期采集了沙蒿的气生部分,采用克莱文格尔法提取精油。为了确定所获得的莎草精油样本的成分组成,采用了色谱-质谱分析法。使用 NIST08 和 FFNSC 质谱库对成分进行鉴定。在精油样本中总共鉴定出 55 种化合物,其中单萜类化合物占 59.92 % - 75.98 %,倍半萜类化合物占 23.24 % - 37.81 %。同时,还发现了 15 种主要化合物。因此,需要注意的是,单萜化合物的含量范围很广:β-蒎烯、反式-β-ocimene、顺式-alloocimene、苯、2,4-戊二烯;倍半萜化合物的含量范围很广:α-copaene、β-sesquiphilandrene、spathulenol。研究样本中精油的定量产量从风干原料的 0.68 % 到 1.36 % 不等,这取决于物种的生长位置。根据所研究精油的成分组成,通过聚类分析确定了 A. salsoloides 的四个独立种群。首次研究了达吉斯坦野生莎草样品气生部分的精油成分取决于环境的非生物因素(海拔高度)。关于莎草精油成分的研究结果表明,莎草精油中含有许多有价值的化合物,可以作为精油的来源。
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