Study of Tildox AVZ effect on rat blood parameters in a subchronic experiment

Abstract

The purpose of our research was to study the effect of Tildox AVZ on rat blood counts in a subchronic experiment. Tildox is a complex antibiotic in powder form. One gram of drug contains doxycycline hyclate – 100 mg and tylosin tartrate - 100 mg as active ingredients, sodium benzoate and sucrose as auxiliary substances. The experiment lasted 90 days. The study was conducted on 150 Wistar rats (75 males and 75 females). The body weight of the animals was 190-230 g. During the experiment 5 groups of 30 animals (15 males and 15 females) were formed, three of them were experimental groups, one - control and one – intact. Of the 150 rats involved in the experiment after its completion 100 individuals (50 males and 50 females) were killed for pathomorphological studies of internal organs.To identify the ability to recover and the appearance of resistance to toxic effects the remaining 50 animals (5 males and 5 females from each group) were monitored for 28 days after the experiment.An aqueous solution of the test sample of the drug was administered daily intragastrically using an atraumatic metal probe. The drug was administered to the first experimental group at a dose of 70 mg / kg, the second group - 265 mg / kg, the third group - at a dose of 1000 mg / kg of animal body weight. The control group was in equal conditions and received intragastric saline solution. Intact animals served as a reference. It was found that Tildox AVZ administered intragastrically at doses of 265 and 1000 mg / kg 1 time per day for 90 days has an effect on clinical and biochemical blood parameters. The drug administered at a dose of 70 mg / kg does not cause changes in blood parameters. However when examining the blood of rats 28 days after the recovery period there were no significant changes in clinical and biochemical parameters compared with the control and intact groups.From this it can be concluded that all blood changes had a dose-dependent effect and after stopping the use of the drug the body recovered which indicates the reversibility of the caused shifts in the blood picture.