First Report of Ocellatin-VT from the Skin Secretion of Leptodactylus vastus Lutz (Amphibia: Leptodactylidae)

Q3 Chemistry

Chemistry Pub Date : 2024-04-08 DOI:10.3390/chemistry6020019

Tulíbia Laurindo Silva, Géssica Gomes Barbosa, Carlos José Correia Santana, P.M.G. Paiva, Mariana S. Castro, T. Napoleão

{"title":"First Report of Ocellatin-VT from the Skin Secretion of Leptodactylus vastus Lutz (Amphibia: Leptodactylidae)","authors":"Tulíbia Laurindo Silva, Géssica Gomes Barbosa, Carlos José Correia Santana, P.M.G. Paiva, Mariana S. Castro, T. Napoleão","doi":"10.3390/chemistry6020019","DOIUrl":null,"url":null,"abstract":"The global emergency of antimicrobial resistance has drawn several efforts to evaluate new drug candidates, such as natural defensive biomolecules. Ocellatins are a group of antimicrobial peptides found in anurans of the Leptodactylidae family. This work investigated the presence of antimicrobial peptides in the skin secretion of Leptodactylus vastus from the Brazilian northeast. The secretion was fractionated by RP-HPLC, and the fractions were screened for antibacterial activity. A peptide isolated from the most active fraction was characterized for primary structure and evaluated for antibacterial activity, cytotoxicity to murine melanoma cells (B16-F10), and hemolytic activity. The RP-HPLC profile displayed 26 fractions, with fraction 25 being the most active. One of the two peptides present in this fraction had the primary structure determined, belonging to the group of ocellatins. Since it was not identical to other ocellatins previously reported, it was named ocellatin-VT. This peptide especially inhibited Gram-negative bacteria growth, with the highest activity against Acinetobacter baumannii and Escherichia coli (growth inhibition was higher than 95% at 8 and 16 µM, respectively). Ocellatin-VT was weakly cytotoxic to B16-F10 cells and showed low hemolytic activity. In conclusion, a new ocellatin was isolated from L. vastus skin secretion that was active against non-resistant and multidrug-resistant bacteria.","PeriodicalId":9850,"journal":{"name":"Chemistry","volume":"284 6","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemistry","FirstCategoryId":"1089","ListUrlMain":"https://doi.org/10.3390/chemistry6020019","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Chemistry","Score":null,"Total":0}

引用次数: 0

Abstract

The global emergency of antimicrobial resistance has drawn several efforts to evaluate new drug candidates, such as natural defensive biomolecules. Ocellatins are a group of antimicrobial peptides found in anurans of the Leptodactylidae family. This work investigated the presence of antimicrobial peptides in the skin secretion of Leptodactylus vastus from the Brazilian northeast. The secretion was fractionated by RP-HPLC, and the fractions were screened for antibacterial activity. A peptide isolated from the most active fraction was characterized for primary structure and evaluated for antibacterial activity, cytotoxicity to murine melanoma cells (B16-F10), and hemolytic activity. The RP-HPLC profile displayed 26 fractions, with fraction 25 being the most active. One of the two peptides present in this fraction had the primary structure determined, belonging to the group of ocellatins. Since it was not identical to other ocellatins previously reported, it was named ocellatin-VT. This peptide especially inhibited Gram-negative bacteria growth, with the highest activity against Acinetobacter baumannii and Escherichia coli (growth inhibition was higher than 95% at 8 and 16 µM, respectively). Ocellatin-VT was weakly cytotoxic to B16-F10 cells and showed low hemolytic activity. In conclusion, a new ocellatin was isolated from L. vastus skin secretion that was active against non-resistant and multidrug-resistant bacteria.

查看原文本刊更多论文

首次报告从 Leptodactylus vastus Lutz（两栖类：Leptodactylidae）皮肤分泌物中发现 Ocellatin-VT

全球抗菌药耐药性的紧急状况促使人们努力评估新的候选药物，如天然防御性生物大分子。Ocellatins 是一类抗菌肽，存在于无尾目猫科动物体内。这项研究调查了巴西东北部巨蜥皮肤分泌物中抗菌肽的存在情况。通过 RP-HPLC 对分泌物进行了分馏，并对分馏物进行了抗菌活性筛选。从活性最强的馏分中分离出的肽具有一级结构特征，并对其抗菌活性、对小鼠黑色素瘤细胞（B16-F10）的细胞毒性和溶血活性进行了评估。RP-HPLC 图谱显示有 26 个馏分，其中馏分 25 的活性最高。该馏分中的两个肽段之一的一级结构已经确定，属于卵球蛋白类。由于它与之前报道的其他卵球蛋白不相同，因此被命名为卵球蛋白-VT。这种多肽特别能抑制革兰氏阴性菌的生长，其中对鲍曼不动杆菌和大肠杆菌的活性最高（8 µM和16 µM时的生长抑制率分别高于95%）。Ocellatin-VT 对 B16-F10 细胞的细胞毒性较弱，溶血活性较低。总之，从大叶黄杨的皮肤分泌物中分离出了一种新的卵白蛋白，它对非耐药和多重耐药细菌都有活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Chemistry

CiteScore

2.50

自引率

0.00%

发文量

审稿时长

11 weeks

期刊介绍： Chemistry—A European Journal is a truly international journal with top quality contributions (2017 ISI Impact Factor: 5.16). It publishes a wide range of outstanding Reviews, Minireviews, Concepts, Full Papers, and Communications from all areas of chemistry and related fields.