Development of Method for Determining Topiramate in Various Biological Matrices (Plasma, Saliva, Hair) and Its Application in Clinical Practice

J. Kuczyńska, Alicja Zakrzewska-Sito, Anna Bochyńska, Halina Sienkiewicz-Jarosz, M. Dermanowski, Paweł Mierzejewski
{"title":"Development of Method for Determining Topiramate in Various Biological Matrices (Plasma, Saliva, Hair) and Its Application in Clinical Practice","authors":"J. Kuczyńska, Alicja Zakrzewska-Sito, Anna Bochyńska, Halina Sienkiewicz-Jarosz, M. Dermanowski, Paweł Mierzejewski","doi":"10.32383/appdr/186004","DOIUrl":null,"url":null,"abstract":"The aim of this work was to develop and validate of method for the determination of topiramate (TPM) by a high-performance liquid chromatographic method coupled to triple-quadrupole mass spectrometry (LC-MS/MS) in MRM mode in the human plasma, saliva, and hair, and implementation of this method for the determination of TPM in patients with epilepsy. Saliva may as an alternative matrix for monitoring drug level, and hair drug content may be a reliable biomarker of the history of drug exposure, allowing to assess patient long-term compliance. Chromatographic separation was achieved in 3 min on a Kinetex analytical column (5 μm C18 100 Å, 100×2.1 mm) using an isocratic elution of acetonitrile and 10 mM ammonium acetate at a ratio of 80:20 (v/v) and a flow rate of 0.2 mL/min. Detection of TPM and internal standard (IS) (TPM-d12) was performed in negative ion mode (ESI−). The following transitions were used m/z 338 → 77.90; 338 → 95.90 for TPM and 350.3 → 78.20 for IS. The method showed to be selective, accurate, precise, and linear for TPM over the concentration ranges of 0.20-30 μg/mL (plasma, saliva), and 5.0-500.0 ng/mL (hair). The simple and robust LC-MS/MS method was successfully applied for the determination of TPM in patients with epilepsy.","PeriodicalId":7135,"journal":{"name":"Acta Poloniae Pharmaceutica - Drug Research","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Poloniae Pharmaceutica - Drug Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.32383/appdr/186004","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

The aim of this work was to develop and validate of method for the determination of topiramate (TPM) by a high-performance liquid chromatographic method coupled to triple-quadrupole mass spectrometry (LC-MS/MS) in MRM mode in the human plasma, saliva, and hair, and implementation of this method for the determination of TPM in patients with epilepsy. Saliva may as an alternative matrix for monitoring drug level, and hair drug content may be a reliable biomarker of the history of drug exposure, allowing to assess patient long-term compliance. Chromatographic separation was achieved in 3 min on a Kinetex analytical column (5 μm C18 100 Å, 100×2.1 mm) using an isocratic elution of acetonitrile and 10 mM ammonium acetate at a ratio of 80:20 (v/v) and a flow rate of 0.2 mL/min. Detection of TPM and internal standard (IS) (TPM-d12) was performed in negative ion mode (ESI−). The following transitions were used m/z 338 → 77.90; 338 → 95.90 for TPM and 350.3 → 78.20 for IS. The method showed to be selective, accurate, precise, and linear for TPM over the concentration ranges of 0.20-30 μg/mL (plasma, saliva), and 5.0-500.0 ng/mL (hair). The simple and robust LC-MS/MS method was successfully applied for the determination of TPM in patients with epilepsy.
开发测定各种生物基质(血浆、唾液、毛发)中托吡酯的方法及其在临床实践中的应用
这项研究的目的是开发并验证在人体血浆、唾液和毛发中采用高效液相色谱法结合三重四极杆质谱(LC-MS/MS)在MRM模式下测定托吡酯(TPM)的方法,并将该方法用于癫痫患者TPM的测定。唾液可作为监测药物水平的替代基质,而毛发中的药物含量可作为药物暴露史的可靠生物标志物,从而评估患者的长期依从性。在 Kinetex 分析柱(5 μm C18 100 Å,100×2.1 mm)上用乙腈和 10 mM 乙酸铵以 80:20 (v/v) 的比例进行等度洗脱,流速为 0.2 mL/min,在 3 分钟内实现色谱分离。在负离子模式(ESI-)下检测 TPM 和内标 (IS)(TPM-d12)。使用以下跃迁 m/z 338 → 77.90;338 → 95.90 检测 TPM,350.3 → 78.20 检测 IS。该方法在 0.20-30 μg/mL(血浆、唾液)和 5.0-500.0 ng/mL(毛发)的浓度范围内对 TPM 具有良好的选择性、准确性、精密度和线性。这种简便、稳健的 LC-MS/MS 方法被成功应用于癫痫患者体内 TPM 的检测。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信