Effect of Chemical and Microbial Additives on Fermentation Profile, Chemical Composition, and Microbial Populations of Whole-Plant Soybean Silage

J. Gandra, C. Takiya, Tiago Antonio Del Valle, C. A. Pedrini, E. Gandra, G. Antonio, E. R. De Oliveira, Igor Kieling Severo, F. Rennó
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Abstract

This study evaluated the effects of two chemical additives or a microbial inoculant on chemical composition and DM losses in whole-plant soybean silage. One-hundred and twenty mini-silos were used in a completely randomized design experiment with the following treatments: water without chloride (control, CON); a microbial inoculant (INO); a chemical additive containing 35–45% formic acid (FA type); and another chemical additive containing 50–60% propionic acid (PA type). Data were analyzed using mixed models of SAS, and treatment differences were evaluated by the following orthogonal contrasts: C1 = CON vs. additives (INO + FA type + PA type); C2 = INO vs. chemical additives (FA type + PA type); and C3 = PA type vs. FA type. Silage pH and ammonia nitrogen concentration were decreased, and concentrations of lactic acid and acetic acid were increased with additives. Counts of lactic acid bacteria were higher in silages with INO than with chemical additives. DM recovery increased with FA type and PA type. Additives increased DM and CP concentrations. Silage A-fraction proportion was greater with additives. Additives, particularly FA type and PA type, improved chemical composition and fermentative profile and reduced undigestible proportions of protein in whole-plant soybean silage. Chemical additives were more effective in reducing silage DM losses than INO.
化学和微生物添加剂对全株大豆青贮发酵概况、化学成分和微生物种群的影响
本研究评估了两种化学添加剂或一种微生物接种剂对全株青贮大豆化学成分和 DM 损失的影响。在完全随机设计实验中使用了 120 株小青贮,处理如下:不含氯化物的水(对照组,CON);微生物接种剂(INO);含 35-45% 甲酸的化学添加剂(FA 型);以及含 50-60% 丙酸的另一种化学添加剂(PA 型)。数据采用 SAS 混合模型进行分析,处理差异通过以下正交对比进行评估:C1 = CON vs. 添加剂(INO + FA 型 + PA 型);C2 = INO vs. 化学添加剂(FA 型 + PA 型);C3 = PA 型 vs. FA 型。添加添加剂后,青贮的 pH 值和氨氮浓度降低,乳酸和乙酸浓度升高。添加 INO 的青贮饲料中乳酸菌数量高于添加化学添加剂的青贮饲料。DM回收率随FA类型和PA类型的增加而增加。添加剂增加了DM和CP浓度。添加添加剂的青贮 A 部分比例更高。添加剂,尤其是 FA 和 PA 类添加剂,可改善全株大豆青贮的化学成分和发酵状况,并降低蛋白质的不可消化比例。在减少青贮 DM 损失方面,化学添加剂比 INO 更有效。
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