Sitti Aisya, Megawati Megawati, Novita Ariani, S. Sukirno, M. Minarti, H. Kurniawan, Arief Hidayat, Medi Hendra, G. Primahana, Akhmad Darmawan
{"title":"ISOLATION AND IDENTIFICATION OF APIGENIN, A FLAVONOID COMPOUND FROM MACARANGA HYPOLEUCA (REICHB.F. & ZOLL.)","authors":"Sitti Aisya, Megawati Megawati, Novita Ariani, S. Sukirno, M. Minarti, H. Kurniawan, Arief Hidayat, Medi Hendra, G. Primahana, Akhmad Darmawan","doi":"10.55981/beritabiologi.2024.3242","DOIUrl":null,"url":null,"abstract":"The study on Macarang hypolueca (Reichb.f. & Zoll.), which was collected from secondary forests around Samarinda City, East Kalimantan, involved phytochemical investigations that led to the isolation of a flavone type compound of flavonoid from the ethyl acetate fraction. To separate the compounds, silica gel column chromatography was utilized with a gradient solvent system of n-hexane and ethyl acetate, along with the addition of 5%. Infrared analysis (FTIR), mass spectrum (LC-ESI-MS), and nuclear magnetic resonance (1D- and 2D-NMR) were used to identify and elucidate the structure. Based on spectroscopic data and comparison with appropriate references, the isolated compound was identified as apigenin","PeriodicalId":280065,"journal":{"name":"BERITA BIOLOGI","volume":"23 S51","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"BERITA BIOLOGI","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.55981/beritabiologi.2024.3242","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The study on Macarang hypolueca (Reichb.f. & Zoll.), which was collected from secondary forests around Samarinda City, East Kalimantan, involved phytochemical investigations that led to the isolation of a flavone type compound of flavonoid from the ethyl acetate fraction. To separate the compounds, silica gel column chromatography was utilized with a gradient solvent system of n-hexane and ethyl acetate, along with the addition of 5%. Infrared analysis (FTIR), mass spectrum (LC-ESI-MS), and nuclear magnetic resonance (1D- and 2D-NMR) were used to identify and elucidate the structure. Based on spectroscopic data and comparison with appropriate references, the isolated compound was identified as apigenin