ISOLATION AND IDENTIFICATION OF APIGENIN, A FLAVONOID COMPOUND FROM MACARANGA HYPOLEUCA (REICHB.F. & ZOLL.)

Sitti Aisya, Megawati Megawati, Novita Ariani, S. Sukirno, M. Minarti, H. Kurniawan, Arief Hidayat, Medi Hendra, G. Primahana, Akhmad Darmawan
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Abstract

The study on Macarang hypolueca (Reichb.f. & Zoll.), which was collected from secondary forests around Samarinda City, East Kalimantan, involved phytochemical investigations that led to the isolation of a flavone type compound of flavonoid from the ethyl acetate fraction. To separate the compounds, silica gel column chromatography was utilized with a gradient solvent system of n-hexane and ethyl acetate, along with the addition of 5%. Infrared analysis (FTIR), mass spectrum (LC-ESI-MS), and nuclear magnetic resonance (1D- and 2D-NMR) were used to identify and elucidate the structure. Based on spectroscopic data and comparison with appropriate references, the isolated compound was identified as apigenin
从 macaranga hypoleuca (reichb.f. & zoll.)中分离和鉴定芹菜素--一种黄酮类化合物
对采集自东加里曼丹州三马林达市附近次生林的Macarang hypolueca (Reichb.f. & Zoll.)进行的研究涉及植物化学调查,结果从乙酸乙酯馏分中分离出一种黄酮类化合物。为了分离这些化合物,采用了硅胶柱色谱法,以正己烷和乙酸乙酯为梯度溶剂系统,同时添加 5%的乙酸乙酯。红外分析(傅立叶变换红外光谱)、质谱(LC-ESI-MS)和核磁共振(一维和二维核磁共振)被用来鉴定和阐明化合物的结构。根据光谱数据以及与相关参考文献的比较,确定分离出的化合物为芹菜甙元
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