Mycotic Granuloma on the Skin of a Desi Fowl: A Case Report

Abstract

Background: A Desi fowl with a nodular growth on the skin of the left lateral aspect of the breast region was presented to VCC, NTR CVSC, Gannavaram. The mass was excised surgically and collected for histopathology and ultrastructural studies. Grossly, the tissue appeared as a white nodular growth, but was cheesy on the cut section. Methods: Fixation and processing of tissue growth for histopathology was done as per routine procedures. The special stains like PAS was carried out to demonstrate the fungal hyphae. Scanning electron microscopy was used to determine the morphology of fungal hyphae. Result: Histopathological sections revealed caseous necrotic centres with numerous fungal hyphae surrounded by polymorphonuclears, macrophages and Langerhans giant cells. Fungal hyphae in tissue sections measured 9-10 µm on micrometry indicating zygomycetes phylum. These pyogranulomatous lesions revealed pinkish red, broad, rarely septate fungal hyphae on PAS staining. Branched fungal hyphae along with attached terminal spores to few hyphae were evident on scanning electron microscopy (SEM).