Effect of Laurus nobilis on bacteria and human transforming growth factor-β1

Revista da Associação Médica Brasileira Pub Date : 2024-04-22 DOI:10.1590/1806-9282.20230683

Okan Sancer, Uğur Şahin, E. S. Çetin, Muhammet Yusuf Tepebaşı, Yasemin Cezaroğlu, Göksel Bilir, Sibel Yünlü, Ahmet Koca

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Abstract

SUMMARY OBJECTIVE: In this study, we aimed to determine the phenolic compounds, the antibacterial activity of extract from Laurus nobilis leaves, and its possible effect on transforming growth factor-β1 expression level in peripheral blood mononuclear cells. METHODS: The phenolic components of Laurus nobilis were identified by the high-performance liquid chromatography method. The antibacterial activity of this extract was determined by disk diffusion and broth microdilution methods. The transforming growth factor-β1 expression was analyzed using the RT-qPCR method. RESULTS: Epicatechin was found in the highest amount and o-coumaric acid in the lowest amount. The half-maximal inhibitory concentration (IC50) was determined to be 55.17 μg/mL. The zones of inhibition and minimum inhibitory concentration for Staphylococcus aureus, Enterococcus faecalis, and Klebsiella pneumoniae were 15, 14, and 8 mm and 125, 250, and 1000 μg/mL, respectively. The change in transforming growth factor-β1 expression levels was found to be statistically significant compared with the control groups (p<0.0001). CONCLUSION: Laurus nobilis extract was found to be effective against bacteria and altered the expression level of transforming growth factor-β1 in peripheral blood mononuclear cells.

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月桂对细菌和人类转化生长因子-β1 的影响

摘要目的：本研究旨在确定月桂叶提取物的酚类化合物、抗菌活性及其对外周血单核细胞中转化生长因子-β1表达水平的可能影响。方法：采用高效液相色谱法鉴定了月桂叶中的酚类成分。采用磁盘扩散法和肉汤微量稀释法测定了该提取物的抗菌活性。采用 RT-qPCR 方法分析了转化生长因子-β1 的表达。结果：表儿茶素的含量最高，邻香豆酸的含量最低。半最大抑制浓度（IC50）为 55.17 μg/mL。金黄色葡萄球菌、粪肠球菌和肺炎克雷伯菌的抑菌区和最小抑菌浓度分别为 15、14 和 8 毫米以及 125、250 和 1000 微克/毫升。与对照组相比，转化生长因子-β1 表达水平的变化具有统计学意义（P<0.0001）。结论：发现月桂提取物对细菌有效，并能改变外周血单核细胞中转化生长因子-β1的表达水平。

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Revista da Associação Médica Brasileira

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