Design of an optical system equipped with blue LEDs for the irradiation of Drosophila melanogaster cultures

IF 1.9 4区 物理与天体物理 Q3 OPTICS
Jorge Enrique Mejía-Sánchez
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引用次数: 0

Abstract

Drosophila melanogaster, better known as the fruit fly, has become a widely used model organism that has allowed us to understand many biological behaviors, from sleep to neurological diseases, behavioral patterns, reproduction, and the circadian cycle, which coordinates biological rhythms in a 24-hour daily cycle through its main Zeitgerber, light, especially blue light. Therefore, the aim of this work was to build an optical setup with a hexagonal design that allowed a large number of Drosophila melanogaster cultures to be irradiated homogeneously with blue light simultaneously. This array can cover an illuminance range from 0 to approximately 600 lux by applying a current variation from 0 to approximately 1 A. It also has a real-time timer to turn the lights on and off, programmed in a 12:12 LD cycle for 24 hours. The optical setup with its unique design can become a very useful tool for developing experiments and understanding paradigms related to blue light at genetic, behavioral and neuronal levels, among others that are still unanswered.
设计配备蓝光 LED 的光学系统,用于照射黑腹果蝇培养物
黑腹果蝇,也就是我们熟知的果蝇,已经成为一种被广泛使用的模式生物,让我们能够了解从睡眠到神经系统疾病、行为模式、繁殖以及昼夜节律周期等许多生物行为,而昼夜节律周期是通过其主要Zeitgerber--光,尤其是蓝光--来协调每天24小时周期内的生物节律。因此,这项工作的目的是建立一个六边形设计的光学装置,使大量的黑腹果蝇培养物能同时接受均匀的蓝光照射。该阵列可通过 0 至约 1 A 的电流变化,覆盖 0 至约 600 lux 的照度范围。它还配有一个实时定时器,可在 24 小时内以 12:12 LD 周期开灯和关灯。该光学装置设计独特,是开发实验和了解与蓝光有关的遗传、行为和神经元水平范例的非常有用的工具,其中包括其他仍未解决的问题。
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来源期刊
CiteScore
2.40
自引率
0.00%
发文量
12
审稿时长
5 weeks
期刊介绍: Rapid progress in optics and photonics has broadened its application enormously into many branches, including information and communication technology, security, sensing, bio- and medical sciences, healthcare and chemistry. Recent achievements in other sciences have allowed continual discovery of new natural mysteries and formulation of challenging goals for optics that require further development of modern concepts and running fundamental research. The Journal of the European Optical Society – Rapid Publications (JEOS:RP) aims to tackle all of the aforementioned points in the form of prompt, scientific, high-quality communications that report on the latest findings. It presents emerging technologies and outlining strategic goals in optics and photonics. The journal covers both fundamental and applied topics, including but not limited to: Classical and quantum optics Light/matter interaction Optical communication Micro- and nanooptics Nonlinear optical phenomena Optical materials Optical metrology Optical spectroscopy Colour research Nano and metamaterials Modern photonics technology Optical engineering, design and instrumentation Optical applications in bio-physics and medicine Interdisciplinary fields using photonics, such as in energy, climate change and cultural heritage The journal aims to provide readers with recent and important achievements in optics/photonics and, as its name suggests, it strives for the shortest possible publication time.
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