Fungal Isolation and Characterization from some Ornamental Plants in Baze University Abuja, Nigeria

F. Mairami, Bello Ahmad
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Abstract

A research was carried out to isolate and characterize the fungal community present on some ornamental plants grown in Baze  University Abuja Nigeria. Leaf samples showing diseased symptoms were collected from four different ornamental plants in the area  (Indian shot, Canna indica; Plantain lily, Hosta undulata; Elephant ear, Caladium sp; and Song of India, Pleomelere flexa. The diseased portions were cut to form an aliquot. Serial dilutions were carried out and the samples were inoculated on Potato Dextrose Agar and  incubated at 27 oC for 5 days. Fungal colonies formed were counted and sub-cultured. Data obtained for occurrence was analyzed in  frequency and percentages while for enumeration using Analysis of Variance (ANOVA) with Duncan’s New Multiple Range Test was used  to separate significant means at 5% level. The result obtained revealed the presence of ten fungal species represented by eight genera  associated with the plants: Aspergillus niger, Aspergillus parasiticus, Mucor racemosus, M. mucedo, Saccharomyces cerevisiae, Fusarium  oxysporum, Rhizopus stolonifer, Alternaria alternata, Pythium aphanidermatum and Penicillium chrysogenum. Aspergillus niger is the  most predominant species. Elephant ear has the highest fungal loads. Four of the fungal species: A. alternata, Aspergillus parasiticus,  Pythium aphanidermatum and Fusarium oxysporum are potential pathogens while the remaining six species are saprophytes. This  implies that the plants are at risk of losing their aesthetic values as such proper attention need to be given to these plants.     
从尼日利亚阿布贾巴泽大学的一些观赏植物中分离真菌并确定其特征
研究人员对尼日利亚阿布贾巴泽大学种植的一些观赏植物上的真菌群落进行了分离和特征描述。研究人员从该地区四种不同的观赏植物(印度射干,Canna indica;车前草,Hosta undulata;象耳,Caladium sp;印度之歌,Pleomelere flexa)上采集了出现病害症状的叶片样本。将患病部分切成等分。对样本进行系列稀释,然后接种到马铃薯葡萄糖琼脂中,在 27 oC 下培养 5 天。对形成的真菌菌落进行计数和亚培养。出现率数据以频率和百分比进行分析,而计数数据则采用方差分析(ANOVA)和邓肯新多重范围检验,以 5%的水平区分显著平均值。结果表明,植物中存在 10 种真菌,分别代表 8 个属:黑曲霉、寄生曲霉、粘孢子菌、粘孢子菌、酿酒酵母菌、氧孢镰刀菌、匍匐根霉、交替孢霉、表皮疫霉和金黄青霉。黑曲霉是最主要的菌种。象耳的真菌负载量最高。其中有 4 种真菌:交替曲霉、寄生曲霉、表皮锈菌和氧孢镰刀菌是潜在的病原体,而其余六种则是营养盐。这意味着这些植物有可能失去其美学价值,因此需要对这些植物给予适当关注。
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