NIFK, a Potential Prognostic and Immunological Biomarker in Pan-- Cancer Analysis, Significantly Regulates Proliferation and Metastasis of Colorectal Cancer.

Zhuoyuan Li, Shangbo Zhou, Ting Bin, Yuntao Shi, Leli Zeng, Bo Li, Jia Li, Yulong He, Changhua Zhang
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Abstract

BACKGROUND As a binding protein of Ki67, NIFK plays an important role in the mitosis of cells and is closely related to the progression of specific types of tumors. However, there is still a lack of systematic analysis of NIFK in pan-cancer and insufficient research to explore its role in human tumors. METHODS We systematically evaluated the pan-cancer expression and mutation of NIFK in human cancers using data from The Cancer Genome Atlas (TCGA) through large-scale bioinformatics analysis. In addition, we explored the pan-cancer immunological characteristics of NIFK, especially in colorectal adenocarcinoma (COAD). Furthermore, we used single-cell sequencing to analyze the expression of NIFK in different cells of COAD tissues and performed GO, KEGG, and gene set enrichment analysis of NIFK in COAD. Lastly, we evaluated the effects of NIFK knockdown on the colorectal cancer cell lines in in vitro experiment. RESULTS We found that NIFK was overexpressed in almost all types of tumors and showed significant prognostic efficacy. Additionally, correlations between NIFK and specific immune features, such as immune cell infiltration, immune checkpoint genes, TMB, and MSI, suggest that NIFK may be used to guide immunotherapy. Subsequently, it was found that the expression of NIFK was significantly upregulated in tumor cells through single-cell sequencing analysis, and the NIFK gene was closely associated with tumor progression and immune therapy response. Finally, we further elucidated the role of NIFK in colorectal cancer and found that downregulation of NIFK expression could inhibit the proliferation, migration, and invasion ability of colorectal cancer cells. CONCLUSION The results of this study demonstrated that NIFK, as a member of the pan-cancer genes, will serve as a biomarker and a potential therapeutic target for a range of cancer types, providing new insight into precision medicine.
NIFK是泛癌症分析中一种潜在的预后和免疫生物标记物,可显著调控结直肠癌的增殖和转移。
背景作为Ki67的结合蛋白,NIFK在细胞有丝分裂过程中发挥着重要作用,并与特定类型肿瘤的进展密切相关。方法 我们利用癌症基因组图谱(The Cancer Genome Atlas,TCGA)中的数据,通过大规模生物信息学分析,系统评估了 NIFK 在人类癌症中的泛癌表达和突变情况。此外,我们还探讨了 NIFK 的泛癌免疫学特征,尤其是在结直肠腺癌(COAD)中的表现。此外,我们还利用单细胞测序分析了NIFK在COAD组织不同细胞中的表达,并对NIFK在COAD中的表达进行了GO、KEGG和基因组富集分析。最后,我们在体外实验中评估了敲除 NIFK 对结直肠癌细胞系的影响。此外,NIFK 与特定免疫特征(如免疫细胞浸润、免疫检查点基因、TMB 和 MSI)之间的相关性表明,NIFK 可用于指导免疫疗法。随后,通过单细胞测序分析发现,NIFK在肿瘤细胞中的表达明显上调,且NIFK基因与肿瘤进展和免疫治疗反应密切相关。最后,我们进一步阐明了 NIFK 在结直肠癌中的作用,发现下调 NIFK 的表达可抑制结直肠癌细胞的增殖、迁移和侵袭能力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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