Synthesis and In vitro Evaluation of Doxorubicin loaded Polymeric Nanoparticles on Cancer Cells

Proceedings of the Pakistan Academy of Sciences: B. Life and Environmental Sciences Pub Date : 2024-04-24 DOI:10.53560/ppasb(61-sp1)1005

Nimra Batool, Tayyaba Saif, Tahira Anwar, Sajjad Ullah, Sikandar Hayat, Malik Ihsan Ullah Khan

{"title":"Synthesis and In vitro Evaluation of Doxorubicin loaded Polymeric Nanoparticles on Cancer Cells","authors":"Nimra Batool, Tayyaba Saif, Tahira Anwar, Sajjad Ullah, Sikandar Hayat, Malik Ihsan Ullah Khan","doi":"10.53560/ppasb(61-sp1)1005","DOIUrl":null,"url":null,"abstract":"Cancer is the second biggest mortality rate globally. Most of anti-cancer drugs are hydrophobic and when they are administered in the body, they get clear from the blood. That’s why polymeric nanoparticles (NPs) have been used for delivering anti-cancer drugs to targeted sites. Biodegradable and self-assembled nature, PEG-PLGA has been used as a nanocarrier for biomedical applications. We developed PEG-PLGA NP for the doxorubicin (DOX) delivery to cancerous cells. The successful PEG-PLGA synthesis was confirmed by its 1 H NMR spectrum. All NPs displayed individual spherical morphology and 100 nm size range with -18.5mV zeta potential. Drug release profile showed DOX had sustained release pattern from DOX@NPs. In vitro, MTT assay and apoptosis analysis revealed that low-dose DOX@NPs exhibited more toxic effects on cancerous cells as compared to DOX alone. Overall results demonstrate that polymeric-based nanosystems increase the efficacy of DOX on cancer cells.","PeriodicalId":20591,"journal":{"name":"Proceedings of the Pakistan Academy of Sciences: B. Life and Environmental Sciences","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Proceedings of the Pakistan Academy of Sciences: B. Life and Environmental Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.53560/ppasb(61-sp1)1005","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Cancer is the second biggest mortality rate globally. Most of anti-cancer drugs are hydrophobic and when they are administered in the body, they get clear from the blood. That’s why polymeric nanoparticles (NPs) have been used for delivering anti-cancer drugs to targeted sites. Biodegradable and self-assembled nature, PEG-PLGA has been used as a nanocarrier for biomedical applications. We developed PEG-PLGA NP for the doxorubicin (DOX) delivery to cancerous cells. The successful PEG-PLGA synthesis was confirmed by its 1 H NMR spectrum. All NPs displayed individual spherical morphology and 100 nm size range with -18.5mV zeta potential. Drug release profile showed DOX had sustained release pattern from DOX@NPs. In vitro, MTT assay and apoptosis analysis revealed that low-dose DOX@NPs exhibited more toxic effects on cancerous cells as compared to DOX alone. Overall results demonstrate that polymeric-based nanosystems increase the efficacy of DOX on cancer cells.

查看原文本刊更多论文

负载多柔比星的聚合物纳米粒子的合成及对癌细胞的体外评估

癌症是全球死亡率第二高的疾病。大多数抗癌药物都是疏水性的，进入人体后会被血液清除。因此，聚合物纳米粒子（NPs）被用于将抗癌药物输送到靶点。PEG-PLGA 具有生物可降解性和自组装性，已被用作生物医学应用的纳米载体。我们开发了 PEG-PLGA NP，用于向癌细胞递送多柔比星（DOX）。其 1 H NMR 光谱证实了 PEG-PLGA 的成功合成。所有 NPs 均呈独立球形，尺寸范围为 100 nm，Zeta 电位为 -18.5mV。药物释放曲线显示 DOX 具有从 DOX@NPs 中持续释放的模式。体外 MTT 试验和细胞凋亡分析表明，与单用 DOX 相比，低剂量 DOX@NPs 对癌细胞的毒性更强。总体结果表明，基于聚合物的纳米系统提高了 DOX 对癌细胞的疗效。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Proceedings of the Pakistan Academy of Sciences: B. Life and Environmental Sciences

自引率

0.00%

发文量