Pramiti Mukhopadhyay, Henry Miller, Aiola Stoja, Alexander J. R. Bishop
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引用次数: 0
Abstract
R-loops are nucleic acid structures composed of a DNA:RNA hybrid with a displaced non-template single-stranded DNA. Current approaches to identify and map R-loop formation across the genome employ either an antibody targeted against R-loops (S9.6) or a catalytically inactivated form of RNase H1 (dRNH1), a nuclease that can bind and resolve DNA:RNA hybrids via RNA exonuclease activity. This overview article outlines several ways to map R-loops using either methodology, explaining the differences and similarities among the approaches. Bioinformatic analysis of R-loops involves several layers of quality control and processing before visualizing the data. This article provides resources and tools that can be used to accurately process R-loop mapping data and explains the advantages and disadvantages of the resources as compared to one another. © 2024 Wiley Periodicals LLC.
绘制和分析 R 循环的方法
R环是一种核酸结构,由DNA:RNA杂交体与移位的非模板单链DNA组成。目前识别和绘制全基因组 R 环形成图谱的方法有两种,一种是针对 R 环的抗体(S9.6),另一种是催化失活的 RNase H1(dRNH1),这种核酸酶能通过 RNA 外切酶活性结合并解析 DNA:RNA 杂交。本文概述了使用这两种方法绘制 R 环的几种方法,并解释了这些方法之间的异同。在对数据进行可视化之前,R-环的生物信息学分析涉及多层质量控制和处理。本文提供了可用于准确处理 R 循环图谱数据的资源和工具,并解释了这些资源相互之间的优缺点。© 2024 Wiley Periodicals LLC.
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