Tinatin Sadunishvili, Dali Gaganidze, Nanuli Amashukeli, Mariam Aznarashvili, Shorena Kharadze, Neli Sturua, Fabio Rezzonico
{"title":"Genetic diversity of Erwinia amylovora isolates from fire blight diseased trees in Central and Eastern Georgia","authors":"Tinatin Sadunishvili, Dali Gaganidze, Nanuli Amashukeli, Mariam Aznarashvili, Shorena Kharadze, Neli Sturua, Fabio Rezzonico","doi":"10.1007/s42161-024-01649-5","DOIUrl":null,"url":null,"abstract":"<p>The genetic diversity of 52 <i>Erwinia amylovora</i> isolates from pome fruit trees with fire blight in Central and Eastern Georgia from the years 2020–2022 was examined using specific PCR and genotyping of CRISPR repeat regions 1 and 2. The analysis identified three distinct genotypes: (A, a, α), matching historical Western European strains; (A, z, α), distinctive for Georgia and differing by a three-spacer deletion in CRR2 (2034–2036); as well as novel genotype (A, ä, α), which was not observed previously. Genotypes (A, a, α) and (A, z, α) were found to coexist geographically in all four regions investigated, whereas genotype (A, ä, α) was reported only in one case in the region of Kvemo Kartli. On separate instances, multiple genotypes were detected even within the same orchard or tree, illustrating the complex genetic landscape of <i>E. amylovora</i> in the country.</p>","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"50 1","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Plant Pathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s42161-024-01649-5","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
The genetic diversity of 52 Erwinia amylovora isolates from pome fruit trees with fire blight in Central and Eastern Georgia from the years 2020–2022 was examined using specific PCR and genotyping of CRISPR repeat regions 1 and 2. The analysis identified three distinct genotypes: (A, a, α), matching historical Western European strains; (A, z, α), distinctive for Georgia and differing by a three-spacer deletion in CRR2 (2034–2036); as well as novel genotype (A, ä, α), which was not observed previously. Genotypes (A, a, α) and (A, z, α) were found to coexist geographically in all four regions investigated, whereas genotype (A, ä, α) was reported only in one case in the region of Kvemo Kartli. On separate instances, multiple genotypes were detected even within the same orchard or tree, illustrating the complex genetic landscape of E. amylovora in the country.
期刊介绍：
The Journal of Plant Pathology (JPP or JPPY) is the main publication of the Italian Society of Plant Pathology (SiPAV), and publishes original contributions in the form of full-length papers, short communications, disease notes, and review articles on mycology, bacteriology, virology, phytoplasmatology, physiological plant pathology, plant-pathogeninteractions, post-harvest diseases, non-infectious diseases, and plant protection. In vivo results are required for plant protection submissions. Varietal trials for disease resistance and gene mapping are not published in the journal unless such findings are already employed in the context of strategic approaches for disease management. However, studies identifying actual genes involved in virulence are pertinent to thescope of the Journal and may be submitted. The journal highlights particularly timely or novel contributions in its Editors’ choice section, to appear at the beginning of each volume. Surveys for diseases or pathogens should be submitted as "Short communications".
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