UV-C treatment elicits resistant responses against Botrytis cinerea infection and the improvement of fruit characteristics in grapevines

IF 2.4 3区 农林科学 Q1 Agricultural and Biological Sciences
Srinivasan Ramalingam, Zar Le Myint, Soon Young Ahn, Jung A. Ryu, Sang-min Lee, Hae Keun Yun
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引用次数: 0

Abstract

UV-C exhibits efficient growth inhibition against a wide range of microorganisms and has an elicitor impact on the induction of resistance against pathogens in host plants, emerging as a promising alternative to fungicides. This study examined the defense elicitor effect of both low (0.05 W/cm2) and high (0.133 W/cm2) powered UV-C (275 nm) against Botrytis cinerea on grapevines. Gene expression, total stilbene content, and the quality of the berries were assessed in ‘Kyoho’ grapevines irradiated with UV-C. Low and high-powered UV-C reduced the in vitro growth of B. cinerea by 40–50% and lowered the in vivo infection and disease severity by 40–85% in the leaves. Lesion formation was reduced by 20–50% in berries treated with UV-C. The spores of B. cinerea did not infect the unwounded berries of ‘Shine Muscat’ and ‘Kyoho’ grape treated with UV-C. The augmented levels of total soluble solids, color values, and reduced titratable acidity improved the quality of the UV-C-irradiated ‘Kyoho’ berries compared to the control. The total stilbene content was four to five times higher with 37.48 µg g−1 fresh weight (FW) using low UV-powered UV-C and 43.11 µg g−1 FW using high UV-powered UV-C in ‘Kyoho’ berry skins treated with UV-C compared to the control (9.24 µg g−1 FW), with predominant levels of trans-resveratrol. The genes involved in stilbene synthesis, defense, and antioxidant activity were strongly upregulated in the leaves and berries of grapevines in response to the low and high UV-C treatments. The low and high UV-C treatments elicit the induction of resistances in grapes against B. cinerea and improve the quality of berries. Future research will be needed on other parameters before UV-C irradiation can be applied to inhibit the incidence of grey mold in vineyards.

Abstract Image

紫外线-C 处理可激发葡萄树对灰葡萄孢菌感染的抗性反应并改善果实特性
紫外线-C 能有效抑制多种微生物的生长,并能诱导寄主植物对病原体产生抗性,是一种很有前途的杀菌剂替代品。本研究考察了低功率(0.05 W/cm2)和高功率(0.133 W/cm2)紫外线-C(275 nm)对葡萄藤上灰葡萄孢菌的防御诱导作用。对接受紫外线-C 照射的 "Kyoho "葡萄树的基因表达、总石烯含量和浆果质量进行了评估。低功率和高功率紫外线-C 可使灰葡萄孢菌的体外生长减少 40-50%,并使叶片的体内感染和病害严重程度降低 40-85%。经紫外线-C 处理的浆果中,病斑的形成减少了 20-50%。用紫外线-C 处理过的 "Shine Muscat "和 "Kyoho "葡萄未受伤的浆果不会受到 B. cinerea 孢子的感染。与对照组相比,紫外线-C 照射下的'Kyoho'浆果的总可溶性固形物含量、色值和可滴定酸度都有所提高。与对照组(9.24 µg g-1 FW)相比,经紫外线-C 处理的'Kyoho'浆果表皮的总芪含量高出四至五倍,使用低紫外线-C 功率时为 37.48 µg g-1 鲜重(FW),使用高紫外线-C 功率时为 43.11 µg g-1 鲜重(FW),其中主要是反式白藜芦醇。在低紫外线-C 和高紫外线-C 处理下,葡萄叶片和浆果中参与二苯乙烯合成、防御和抗氧化活性的基因强烈上调。低紫外线-C 和高紫外线-C 处理可诱导葡萄对灰葡萄孢菌产生抗性,并改善浆果的质量。在应用紫外线-C 照射抑制葡萄园灰霉病发病率之前,还需要对其他参数进行研究。
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来源期刊
Horticulture Environment and Biotechnology
Horticulture Environment and Biotechnology Agricultural and Biological Sciences-Horticulture
CiteScore
4.30
自引率
4.20%
发文量
0
审稿时长
6 months
期刊介绍: Horticulture, Environment, and Biotechnology (HEB) is the official journal of the Korean Society for Horticultural Science, was launched in 1965 as the "Journal of Korean Society for Horticultural Science". HEB is an international journal, published in English, bimonthly on the last day of even number months, and indexed in Biosys Preview, SCIE, and CABI. The journal is devoted for the publication of original research papers and review articles related to vegetables, fruits, ornamental and herbal plants, and covers all aspects of physiology, molecular biology, biotechnology, protected cultivation, postharvest technology, and research in plants related to environment.
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