The development and utilization of two SCAR markers linked to the resistance of banana (Musa spp. AAA) to Fusarium oxysporum f. sp. cubense race 4

IF 1.6 3区农林科学 Q2 AGRONOMY

Euphytica Pub Date : 2024-04-03 DOI:10.1007/s10681-024-03323-4

Mengran Qv, Guoen Feng, Shule Chen, Houbin Chen, Chengjie Chen, Fang Wang, Shun Lv, Longyu Dai, Hui Liu, Bingzhi Huang, Xiaoquan Li, Zuxiang Su, Chunxiang Xu

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引用次数: 0

Abstract

Banana (Musa spp.) production worldwide is seriously threatened by Fusarium wilt caused by Fusarium oxysporum f. sp. cubense (Foc). The best way to control this disease is to grow resistant cultivars. However, it requires large-scale field evaluations and labor- and time-consuming to obtain disease-resistant germplasm. Development of early, reliable, and reproducible selection strategies are considered as the efficient approach which could speed up the selection procedure. In this study, two pairs of sequence-related amplified polymorphism (SRAP) primers related to banana Foc resistance/susceptibility were screened from 100 pairs of random primers. Correspondingly, two pairs of sequence characterized amplified region (SCAR) markers (namely SC4-F/SC4-R and SC14-F/SC14-R, respectively) were successfully generated from these two SRAP markers using 30 cultivars either resistant or susceptible to Foc. Both SCAR markers were located in mitochondrion genome and showed discriminatory power of 96.67% and 100%, respectively. Mitochondrial proteins possibly play a very important role in banana resistance to Foc. In additional, these two SCAR markers were employed simultaneously to screen potential resistant germplasm from 53 accessions with unknown resistance to Foc, and the results revealed a consistency of 83.0% with each other, further indicating their high reliability and reproducibility. These results suggest that both SCAR markers could be used in molecular marker-assisted selection for banana germplasm resistant to Fusarium.

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与香蕉（Musa spp. AAA）对 Fusarium oxysporum f. sp. cubense race 4 的抗性相关的两个 SCAR 标记的开发和利用

由 Fusarium oxysporum f. sp. cubense（Foc）引起的镰刀菌枯萎病严重威胁着全世界的香蕉（Musa spp.）生产。控制这种病害的最佳方法是培育抗病品种。然而，要获得抗病种质需要进行大规模的田间评估，费工费时。开发早期、可靠、可重复的选择策略被认为是加快选择过程的有效方法。本研究从 100 对随机引物中筛选出两对与香蕉 Foc 抗性/感病性相关的序列相关扩增多态性（SRAP）引物。相应地，利用 30 个对 Foc 具有抗性或敏感性的栽培品种，成功地从这两个 SRAP 标记中产生了两对序列特征扩增区（SCAR）标记（分别为 SC4-F/SC4-R 和 SC14-F/SC14-R）。这两个 SCAR 标记都位于线粒体基因组，其鉴别力分别为 96.67% 和 100%。线粒体蛋白可能在香蕉抵抗 Foc 的过程中起着非常重要的作用。此外，还同时使用这两种 SCAR 标记从 53 个对 Foc 抗性未知的品种中筛选潜在的抗性种质，结果显示它们之间的一致性达到 83.0%，进一步表明它们具有很高的可靠性和可重复性。这些结果表明，这两种 SCAR 标记可用于分子标记辅助筛选抗镰刀菌的香蕉种质。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Euphytica 农林科学-农艺学

CiteScore

3.80

自引率

5.30%

发文量

157

审稿时长

4.5 months

期刊介绍： Euphytica is an international journal on theoretical and applied aspects of plant breeding. It publishes critical reviews and papers on the results of original research related to plant breeding. The integration of modern and traditional plant breeding is a growing field of research using transgenic crop plants and/or marker assisted breeding in combination with traditional breeding tools. The content should cover the interests of researchers directly or indirectly involved in plant breeding, at universities, breeding institutes, seed industries, plant biotech companies and industries using plant raw materials, and promote stability, adaptability and sustainability in agriculture and agro-industries.