Laboratory‐scale preparation of prostaglandins using acetone powder of the red alga Gracilaria vermiculophylla

IF 1.6 4区生物学 Q2 MARINE & FRESHWATER BIOLOGY

Phycological Research Pub Date : 2024-04-08 DOI:10.1111/pre.12548

Muhammad Ikbal Illijas, Nobuya Suzuki, Masaki Honda, Nur Rahmawaty Arma, Andriani Nasir, Luqman Saleh, Dahlia Dahlia, Rahmi Mulyani, Yutaka Itabashi

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引用次数: 0

Abstract

SummaryThe red alga Gracilaria vermiculophylla is a prostaglandin (PG)‐producing macroalga. The alga is rich in polyunsaturated fatty acids with 20 carbon atoms, mainly arachidonic acid (AA), which is a precursor of PGs. The purpose of the present study was to analyze the ability of the red alga to produce PGs using acetone powder as the crude enzyme prepared from the alga. The acetone powder (250 mg) was incubated with different amounts of exogenous AA (0.1–4 mg). For the determination of PG contents, 5 μL of a sample solution (5 mL in water) consisting of acetone powder and AA was injected into the HPLC column. For PG analysis, an HPLC system connected with a mass spectrometer was used. Results of the study showed that the released PGs from incubation of acetone powder and AA consisted of PGE2, 15‐keto‐PGE2, 15‐hydroperoxy‐PGE2, PGA2, and PGF2α. The capability of the crude enzyme prepared from the red alga to produce PGs was affected by available oxygen and AA concentrations. The crude enzyme (250 mg) was capable of producing 164 and 141 μg of PGE2 and 15‐keto‐PGE2, respectively, from incubation with 250 μg of AA. This in vitro method could be a simple way to provide PGs in the laboratory.

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利用红藻蛭石丙酮粉在实验室规模制备前列腺素

摘要红藻蛭石藻（Gracilaria vermiculophylla）是一种生产前列腺素（PG）的大型藻类。该藻富含 20 个碳原子的多不饱和脂肪酸，主要是花生四烯酸（AA），而花生四烯酸是前列腺素的前体。本研究的目的是利用丙酮粉作为从红藻中制备的粗酶，分析红藻生产 PGs 的能力。将丙酮粉（250 毫克）与不同量的外源 AA（0.1-4 毫克）进行培养。为了测定 PG 含量，将 5 μL 由丙酮粉末和 AA 组成的样品溶液（5 mL 水）注入 HPLC 柱。在分析 PG 时，使用了与质谱仪相连的 HPLC 系统。研究结果表明，丙酮粉末和 AA 培养释放的 PG 包括 PGE2、15-酮-PGE2、15-氢过氧-PGE2、PGA2 和 PGF2α。从红藻中制备的粗酶产生 PGs 的能力受可用氧气和 AA 浓度的影响。粗酶（250 毫克）与 250 微克 AA 培养后，能分别产生 164 和 141 微克 PGE2 和 15-酮-PGE2。这种体外方法是在实验室中提供 PGs 的一种简单方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Phycological Research 生物-海洋与淡水生物学

CiteScore

3.60

自引率

13.30%

发文量

审稿时长

>12 weeks

期刊介绍： Phycological Research is published by the Japanese Society of Phycology and complements the Japanese Journal of Phycology. The Journal publishes international, basic or applied, peer-reviewed research dealing with all aspects of phycology including ecology, taxonomy and phylogeny, evolution, genetics, molecular biology, biochemistry, cell biology, morphology, physiology, new techniques to facilitate the international exchange of results. All articles are peer-reviewed by at least two researchers expert in the filed of the submitted paper. Phycological Research has been credited by the International Association for Plant Taxonomy for the purpose of registration of new non-vascular plant names (including fossils).